RT Journal Article
T1 MDR Shigella sonnei in Spain: an ever-evolving emerging threat?
A1 Ortiz-de-la-Rosa, Jose Manuel
A1 Rodriguez-Villodres, Angel
A1 Casimiro-Soriguer, Carlos S
A1 Ruiz-Perez-De-Pipaon, Maite
A1 Briones, Eduardo
A1 Aznar-Fernandez, Maria
A1 Lepe, Jose Antonio
K1 Diarrhea
K1 Cluster Analysis
K1 Hospitals
K1 Mutation
K1 Genomics
AB Seven CTX-M-27-producing Shigella sonnei strains were isolated at the University Hospital Virgen del Rocío (Seville, Spain) microbiology service from October to November 2021. To offer extensive information on the microbiological and molecular epidemiology results of the seven S. sonnei isolates and compare them with other previously documented CTX-M-27-producing S. sonnei associated with MSM transmission. S. sonnei isolated from stool samples of patients with acute diarrhoea were identified through biochemical and serological typing. Whole characterization of the seven isolates was performed by sequencing with MinION Mk1C followed by genomic and molecular analysis. All the isolates were resistant to penicillins, cephalosporins, fluoroquinolones, cotrimoxazole and azithromycin. Sequencing showed the presence of several resistance determinants, outstanding blaCTX-M-27, azithromycin resistance genes [ermB and mph(A)], qnrB19 and mutations in the QRDRs. All isolates belonged to the same hierarchical clustering of cgMLST (HierCC) with five allele distance (HC5) scheme v1 from EnteroBase. However, they presented differences in plasmid composition, with all seven isolates harbouring IncFII, IncB/O/K/Z and ColE1-like while SH2, SH6 and SH7 had IncFIB only. Our isolates were closely related to others from Spain (HC5; 98748), Australia (HC5; 98748) and the UK (HC5; 98748), which were also associated with MSM transmission. Nevertheless, the structure of the non-chromosomal genetic elements and the genetic context of blaCTX-M-27 presented a certain variability compared with isolates from other countries and among them. This study confirms the emergence of CTX-M-27-producing S. sonnei (ST152) associated with MSM transmission in Spain, adding it to the Europe outbreak list and reinforcing the necessity of active surveillance and control of this high-risk clone.
PB Oxford University Press
YR 2022
FD 2022-09-05
LK http://hdl.handle.net/10668/19807
UL http://hdl.handle.net/10668/19807
LA en
NO Ortiz de la Rosa JM, Rodríguez-Villodres Á, Casimiro-Soriguer CS, Ruiz-Pérez De Pipaón M, Briones E, Aznar Fernández M, et al. MDR Shigella sonnei in Spain: an ever-evolving emerging threat? JAC Antimicrob Resist. 2022 Sep 5;4(5):dlac090.
DS RISalud
RD Jul 7, 2025