RT Journal Article
T1 Both p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast cancer.
A1 Galindo-Moreno, Maria
A1 Giraldez, Servando
A1 Saez, Carmen
A1 Japon, Miguel A
A1 Tortolero, Maria
A1 Romero, Francisco
K1 Autophagy
K1 Gene Expression Regulation, Neoplastic
K1 HeLa Cells
K1 Humans
K1 Proteasome Endopeptidase Complex
K1 Protein Stability
K1 SKP Cullin F-Box Protein Ligases
K1 Signal Transduction
AB Cyclin-dependent kinase 1 (CDK1) is the central mammalian regulator of cell proliferation and a promising therapeutic target for breast cancer. In fact, CDK1 inhibition downregulates survival and induces apoptosis. Due to its essential role, CDK1 expression and activity are strictly controlled at various levels. We previously described that CDK1 stability is also regulated and that SCF(βTrCP) ubiquitinates CDK1, which is degraded via the lysosomal pathway. In addition, in breast tumors from patients, we found a negative correlation between CDK1 accumulation and βTrCP levels, and a positive correlation with the degree of tumor malignancy. This prompted us to study the molecular mechanism involved in CDK1 clearance. In this report, we determine that both chemotherapeutic agents and proteolytic stress induce CDK1 degradation in human breast cancer MCF7 cells through p62/HDAC6-mediated selective autophagy. On the one hand, CDK1 binds to p62/SQSTM1-LC3 and, on the other hand, it interacts with HDAC6. Both complexes are dependent on the presence of an intact βTrCP-binding motif on CDK1. Furthermore, we also show that CDK1 is recruited to aggresomes in response to proteasome inhibition for an extended period. We propose CDK1 clearance as a potential predictive biomarker of antitumor treatment efficacy.
PB Nature Publishing Group
YR 2017
FD 2017-08-30
LK http://hdl.handle.net/10668/11545
UL http://hdl.handle.net/10668/11545
LA en
NO Galindo-Moreno M, Giráldez S, Sáez C, Japón MÁ, Tortolero M, Romero F. Both p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast cancer. Sci Rep. 2017 Aug 30;7(1):10078.
DS RISalud
RD Apr 7, 2025