RT Journal Article
T1 Reference Values to Assess Hemodilution and Warn of Potential False-Negative Minimal Residual Disease Results in Myeloma.
A1 Puig, Noemí
A1 Flores-Montero, Juan
A1 Burgos, Leire
A1 Cedena, María-Teresa
A1 Cordón, Lourdes
A1 Pérez, José-Juan
A1 Sanoja-Flores, Luzalba
A1 Manrique, Irene
A1 Rodríguez-Otero, Paula
A1 Rosiñol, Laura
A1 Martínez-López, Joaquín
A1 Mateos, María-Victoria
A1 Lahuerta, Juan-José
A1 Bladé, Joan
A1 San Miguel, Jesús F
A1 Orfao, Alberto
A1 Paiva, Bruno
K1 hemodilution
K1 minimal residual disease
K1 multiple myeloma
AB Whereas, in most patients with multiple myeloma (MM), achieving undetectable MRD anticipates a favorable outcome, some others relapse shortly afterwards. Although one obvious explanation for this inconsistency is the use of nonrepresentative marrow samples due to hemodilution, there is no guidance on how to evaluate this issue. Since B-cell precursors, mast cells and nucleated red blood cells are normally absent in peripheral blood, we analyzed them in 1404 bone marrow (BM) aspirates obtained in numerous disease settings and in 85 healthy adults (HA). First, we confirmed the systematic detection of the three populations in HA, as well as the nonreduced numbers with aging. Pairwise comparisons between HA and MM patients grouped according to age and treatment showed significant variability, suggesting that hemodilution should be preferably evaluated with references obtained from patients treated with identical regimens. Leveraging the MRD results from 118 patients, we showed that a comparison with HA of similar age could also inform on potential hemodilution. Our study supports the routine assessment of BM cellularity to evaluate hemodilution, since reduced BM-specific cell types as compared to reference values (either treatment-specific or from HA if the former are unavailable) could indicate hemodilution and a false-negative MRD result.
SN 2072-6694
YR 2021
FD 2021-09-30
LK https://hdl.handle.net/10668/27840
UL https://hdl.handle.net/10668/27840
LA en
DS RISalud
RD Apr 12, 2025