RT Journal Article
T1 Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes
A1 Montserrat-de la Paz, Sergio
A1 Rodriguez-Martin, Noelia M.
A1 Villanueva, Alvaro
A1 Pedroche, Justo
A1 Cruz-Chamorro, Ivan
A1 Millan, Francisco
A1 Millan-Linares, Maria C.
K1 Wheat gluten
K1 Protein hydrolysates
K1 Atherosclerosis
K1 Monocytes
K1 Celiac disease
K1 Hidrolisados de proteína
K1 Aterosclerosis
K1 Monocitos
K1 Enfermedad celíaca
AB Bioactive protein hydrolysates have been identified in several sources as possible agents in the prevention and treatment of many diseases. A wheat gluten (WG) concentrate was hydrolyzed by Alcalase under specific conditions. The resulting hydrolysates were evaluated by in vitro cell-free experiments leading to the identification of one bioactive WG protein hydrolysate (WGPH), which was used at 50 and 100 μg/mL on primary human monocytes. Reactive oxygen species (ROS) and nitrite levels and RT-qPCR and ELISA techniques were used to analyze the functional activity of WGPH. Our results showed that WGPH hydrolyzed in 45 min (WGPH45A) down-regulated gene expression of Interleukin (IL)-1β, IL-6, IL-17, and Interferon gamma (IFNγ) and reduced cytokine release in lipopolysaccharide (LPS)-stimulated monocytes. In addition, WGPH45A down-regulated gene-related to atherosclerotic onset. Our results suggest that WGPH45A has a potent anti-inflammatory and atheroprotective properties, reducing the expression of gene-related inflammation and atherosclerosis that could be instrumental in maintaining cardiovascular homeostasis.
PB MDPI
YR 2020
FD 2020-06-30
LK http://hdl.handle.net/10668/4076
UL http://hdl.handle.net/10668/4076
LA en
NO Montserrat-de la Paz S, Rodriguez-Martin NM, Villanueva A, Pedroche J, Cruz-Chamorro I, Millan F, et al. Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes. Foods. 2020 Jun 30;9(7):854
DS RISalud
RD Apr 6, 2025