RT Journal Article
T1 Pru p 3-Epitope-based sublingual immunotherapy in a murine model for the treatment of peach allergy
A1 Rodriguez, Maria J.
A1 Mascaraque, Ainhoa
A1 Ramos-Soriano, Javier
A1 Torres, Maria J.
A1 Perkins, James R.
A1 Gomez, Francisca
A1 Garrido-Arandia, Maria
A1 Cubells-Baeza, Nuria
A1 Andreu, David
A1 Diaz-Perales, Araceli
A1 Rojo, Javier
A1 Mayorga, Cristobalina
K1 Anaphylaxis
K1 Food-allergy
K1 Lipid transfer protein
K1 Mouse model
K1 Sublingual immunotherapy
K1 T-cell epitopes
K1 Food allergy
K1 Oral immunotherapy
K1 Cpg oligodeoxynucleotides
K1 Airway inflammation
K1 Dendritic cells
K1 Peanut
K1 Mice
K1 Mechanisms
K1 Sensitization
AB Scope: Food-specific immunotherapy (SIT) is a promising treatment for lipid transfer protein (LTP)-syndrome. We propose a novel sublingual-SIT (SLIT) that combines a Pru p 3 T-cell peptide and an oligodeoxyribonucleotide (ODN) with CpG motifs (ODN-CpG) as adjuvants to induce a specific Th1/Treg response.Methods and results: LTP-peach allergic mice were treated sublingually with a combination of a CpG sequence and mono- or tetravalent systems including a Pru p 3 peptide, D-1(Prup3) or D-4(Prup3). Mice were challenged intraperitoneally with Pru p 3 one or three weeks after SLIT and tolerance was assessed.Mice treated with D-1(Prup3)+CpG were protected from anaphylaxis after Pru p 3 challenge. They showed no change in body temperature, lower levels of Pru p 3-specific IgE and IgG1 antibodies and higher levels of sIgG2a compared to the untreated group. They had fewer IgE and IgG1 secreting cells and more sIgG2a secreting cells. Moreover, a significantly lower number of Pru p 3-specific CD4(+)T cells and a higher number of Treg cells were found, alongside a Th1 cytokine pattern. These changes were maintained for three weeks after stopping treatment.Conclusion: D(1)Prup3+CpG represents a promising SIT for food allergy. It is easily synthesized and induces protection from anaphylaxis to Pru p 3 that is maintained for at least three weeks.
PB Wiley
SN 1613-4125
YR 2017
FD 2017-05-17
LK http://hdl.handle.net/10668/18616
UL http://hdl.handle.net/10668/18616
LA en
NO Rodriguez MJ, Mascaraque A, Ramos-Soriano J, Torres MJ, Perkins JR, Gomez F, et al. Pru p 3-Epitope-based sublingual immunotherapy in a murine model for the treatment of peach allergy. Mol Nutr Food Res. 2017 Oct;61(10)
NO The study was funded by the Institute of Health “Carlos III” of the Ministry of Economy and Competitiveness: PI12/02481 and PI15/00559, RETICS RIRAAF (RD12/0013/0001, RD12/0013/0014, RD12/0013/0016), RETICS ARADyAL(RD16/0006/0001 RD16/0006/0003 and RD16/0006/0011) and Sara Borrell Program (CD14/00242). Andalusian Regional Ministry of Economy and Knowledge: CTS-7433), Nicolas Monardes Program (C-0044-2012 SAS2013) and the Spanish Ministry of Economy and Competitiveness (CTQ2014-52328-Pand AGL2014-52395-C2-2). Grants were cofunded by the European Regional Development Fund (ERDF).
DS RISalud
RD Apr 5, 2025