%0 Journal Article
%A Rodriguez, Maria J.
%A Mascaraque, Ainhoa
%A Ramos-Soriano, Javier
%A Torres, Maria J.
%A Perkins, James R.
%A Gomez, Francisca
%A Garrido-Arandia, Maria
%A Cubells-Baeza, Nuria
%A Andreu, David
%A Diaz-Perales, Araceli
%A Rojo, Javier
%A Mayorga, Cristobalina
%T Pru p 3-Epitope-based sublingual immunotherapy in a murine model for the treatment of peach allergy
%D 2017
%@ 1613-4125
%U http://hdl.handle.net/10668/18616
%X Scope: Food-specific immunotherapy (SIT) is a promising treatment for lipid transfer protein (LTP)-syndrome. We propose a novel sublingual-SIT (SLIT) that combines a Pru p 3 T-cell peptide and an oligodeoxyribonucleotide (ODN) with CpG motifs (ODN-CpG) as adjuvants to induce a specific Th1/Treg response.Methods and results: LTP-peach allergic mice were treated sublingually with a combination of a CpG sequence and mono- or tetravalent systems including a Pru p 3 peptide, D-1(Prup3) or D-4(Prup3). Mice were challenged intraperitoneally with Pru p 3 one or three weeks after SLIT and tolerance was assessed.Mice treated with D-1(Prup3)+CpG were protected from anaphylaxis after Pru p 3 challenge. They showed no change in body temperature, lower levels of Pru p 3-specific IgE and IgG1 antibodies and higher levels of sIgG2a compared to the untreated group. They had fewer IgE and IgG1 secreting cells and more sIgG2a secreting cells. Moreover, a significantly lower number of Pru p 3-specific CD4(+)T cells and a higher number of Treg cells were found, alongside a Th1 cytokine pattern. These changes were maintained for three weeks after stopping treatment.Conclusion: D(1)Prup3+CpG represents a promising SIT for food allergy. It is easily synthesized and induces protection from anaphylaxis to Pru p 3 that is maintained for at least three weeks.
%K Anaphylaxis
%K Food-allergy
%K Lipid transfer protein
%K Mouse model
%K Sublingual immunotherapy
%K T-cell epitopes
%K Food allergy
%K Oral immunotherapy
%K Cpg oligodeoxynucleotides
%K Airway inflammation
%K Dendritic cells
%K Peanut
%K Mice
%K Mechanisms
%K Sensitization
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