RT Journal Article
T1 Microarray profiling of mononuclear peripheral blood cells identifies novel candidate genes related to chemoradiation response in rectal cancer.
A1 Palma, Pablo
A1 Cuadros, Marta
A1 Conde-Muíño, Raquel
A1 Olmedo, Carmen
A1 Cano, Carlos
A1 Segura-Jiménez, Inmaculada
A1 Blanco, Armando
A1 Bueno, Pablo
A1 Ferrón, J Antonio
A1 Medina, Pedro
K1 Neoplasias del Recto
K1 Quimioradioterapia
K1 Análisis de Secuencia por Matrices de Oligonucleótidos
K1 Cuidados Preoperatorios
K1 Resultado del Tratamiento
AB Preoperative chemoradiation significantly improves oncological outcome in locally advanced rectal cancer. However there is no effective method of predicting tumor response to chemoradiation in these patients. Peripheral blood mononuclear cells have emerged recently as pathology markers of cancer and other diseases, making possible their use as therapy predictors. Furthermore, the importance of the immune response in radiosensivity of solid organs led us to hypothesized that microarray gene expression profiling of peripheral blood mononuclear cells could identify patients with response to chemoradiation in rectal cancer. Thirty five 35 patients with locally advanced rectal cancer were recruited initially to perform the study. Peripheral blood samples were obtained before neaodjuvant treatment. RNA was extracted and purified to obtain cDNA and cRNA for hybridization of microarrays included in Human WG CodeLink bioarrays. Quantitative real time PCR was used to validate microarray experiment data. Results were correlated with pathological response, according to Mandard´s criteria and final UICC Stage (patients with tumor regression grade 1-2 and downstaging being defined as responders and patients with grade 3-5 and no downstaging as non-responders). Twenty seven out of 35 patients were finally included in the study. We performed a multiple t-test using Significance Analysis of Microarrays, to find those genes differing significantly in expression, between responders (n = 11) and non-responders (n = 16) to CRT. The differently expressed genes were: BC 035656.1, CIR, PRDM2, CAPG, FALZ, HLA-DPB2, NUPL2, and ZFP36. The measurement of FALZ (p = 0.029) gene expression level determined by qRT-PCR, showed statistically significant differences between the two groups. Gene expression profiling reveals novel genes in peripheral blood samples of mononuclear cells that could predict responders and non-responders to chemoradiation in patients with locally advanced rectal cancer. Moreover, our investigation added further evidence to the importance of mononuclear cells' mediated response in the neoadjuvant treatment of rectal cancer.
PB Public Library of Science
YR 2013
FD 2013-09-05
LK http://hdl.handle.net/10668/1396
UL http://hdl.handle.net/10668/1396
LA en
NO Palma P, Cuadros M, Conde-Muíño R, Olmedo C, Cano C, Segura-Jiménez I, et al. Microarray profiling of mononuclear peripheral blood cells identifies novel candidate genes related to chemoradiation response in rectal cancer. PLoS ONE. 2013; 8(9):e74034
NO Journal Article;
DS RISalud
RD Apr 17, 2025