RT Journal Article
T1 Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves.
A1 El Soury, Marwa
A1 Garcia-Garcia, Oscar Dario
A1 Moretti, Matteo
A1 Perroteau, Isabelle
A1 Raimondo, Stefania
A1 Lovati, Arianna Barbara
A1 Carriel, Víctor
K1 acellular
K1 decellularization
K1 extracellular matrix
K1 orthopedic trauma
K1 peripheral nerves
K1 regeneration
AB In critical nerve gap repair, decellularized nerve allografts are considered a promising tissue engineering strategy that can provide superior regeneration results compared to nerve conduits. Decellularized nerves offer a well-conserved extracellular matrix component that has proven to play an important role in supporting axonal guiding and peripheral nerve regeneration. Up to now, the known decellularized techniques are time and effort consuming. The present study, performed on rat sciatic nerves, aims at investigating a novel nerve decellularization protocol able to combine an effective decellularization in short time with a good preservation of the extracellular matrix component. To do this, a decellularization protocol proven to be efficient for tendons (DN-P1) was compared with a decellularization protocol specifically developed for nerves (DN-P2). The outcomes of both the decellularization protocols were assessed by a series of in vitro evaluations, including qualitative and quantitative histological and immunohistochemical analyses, DNA quantification, SEM and TEM ultrastructural analyses, mechanical testing, and viability assay. The overall results showed that DN-P1 could provide promising results if tested in vivo, as the in vitro characterization demonstrated that DN-P1 conserved a better ultrastructure and ECM components compared to DN-P2. Most importantly, DN-P1 was shown to be highly biocompatible, supporting a greater number of viable metabolically active cells.
PB MDPI AG
YR 2021
FD 2021-02-24
LK http://hdl.handle.net/10668/17313
UL http://hdl.handle.net/10668/17313
LA en
NO El Soury M, García-García ÓD, Moretti M, Perroteau I, Raimondo S, Lovati AB, et al. Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves. Int J Mol Sci. 2021 Feb 27;22(5):2389.
NO This research was partially supported by: the Italian Ministry of Health “Ricerca Corrente”;the Fondazione Cassa di Risparmio di Torino (Turin, Italy), protocol number 2017.AI190.U219,RF: 2016.2388; the Spanish “Plan Nacional de Investigación Científica, Desarrollo e InnovaciónTecnológica, Ministerio de Economía y Competitividad (Instituto de Salud Carlos III)”, Grants NoFIS PI17-0393 and FIS PI20-0318, co-financed by the “Fondo Europeo de Desarrollo Regional ERDFFEDER European Union”; Grant No P18-RT-5059 by “Plan Andaluz de Investigación, Desarrollo eInnovación (PAIDI 2020), Consejería de Transformación Económica, Industria, Conocimiento yUniversidades, Junta de Andalucía, España”; and Grant A-CTS-498-UGR18 by “Programa OperativoFEDER Andalucía 2014-2020, Universidad de Granada, Junta de Andalucía, España”, co-funded byERDF-FEDER, the European Union.
DS RISalud
RD Apr 8, 2025