RT Journal Article
T1 Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria.
A1 Vila Domínguez, Andrea
A1 Ayerbe Algaba, Rafael
A1 Miró Canturri, Andrea
A1 Rodríguez Villodres, Ángel
A1 Smani, Younes
K1 Gram-negative bacteria
K1 Gram-positive bacteria
K1 colloidal silver
K1 multidrug-resistant bacteria
AB Due to the emergence of antimicrobial resistance, new alternative therapies are needed. Silver was used to treat bacterial infections since antiquity due to its known antimicrobial properties. Here, we aimed to evaluate the in vitro activity of colloidal silver (CS) against multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria. A total of 270 strains (Acinetobacter baumannii (n = 45), Pseudomonas aeruginosa (n = 25), Escherichia coli (n = 79), Klebsiella pneumoniae (n = 58)], Staphylococcus aureus (n = 34), Staphylococcus epidermidis (n = 14), and Enterococcus species (n = 15)) were used. The minimal inhibitory concentration (MIC) of CS was determined for all strains by using microdilution assay, and time-kill curve assays of representative reference and MDR strains of these bacteria were performed. Membrane permeation and bacterial reactive oxygen species (ROS) production were determined in presence of CS. CS MIC90 was 4-8 mg/L for all strains. CS was bactericidal, during 24 h, at 1× and 2× MIC against Gram-negative bacteria, and at 2× MIC against Gram-positive bacteria, and it did not affect their membrane permeabilization. Furthermore, we found that CS significantly increased the ROS production in Gram-negative with respect to Gram-positive bacteria at 24 h of incubation. Altogether, these results suggest that CS could be an effective treatment for infections caused by MDR Gram-negative and Gram-positive bacteria.
SN 2079-6382
YR 2020
FD 2020-01-19
LK https://hdl.handle.net/10668/28026
UL https://hdl.handle.net/10668/28026
LA en
DS RISalud
RD Apr 17, 2025