RT Journal Article
T1 Cytotoxicity, Epidermal Barrier Function and Cytokine Evaluation after Antiseptic Treatment in Bioengineered Autologous Skin Substitute.
A1 Garcia-Valdivia, Marta
A1 Quiñones-Vico, Maria I
A1 Ortega-Llamas, Laura
A1 Fernandez-Gonzalez, Ana
A1 Ubago-Rodriguez, Ana
A1 Sanabria-de la Torre, Raquel
A1 Arias-Santiago, Salvador
K1 bioengineered autologous skin substitute
K1 cell viability
K1 cytokine secretion
K1 drug development
K1 epidermal barrier function
K1 in vitro model
K1 regenerative medicine
K1 tissue engineering
AB Bioengineered autologous skin substitutes (BASS) technology is an emerging field for skin burn therapy. However, further studies on BASS characterization, viability against standard procedures for wound healing, and protocol optimization are necessary for the improvement of BASS technology for clinical use. The aim of this study is to evaluate the effect of common antiseptics for clinical use in BASS, focusing on cell viability, inflammatory cytokine pattern, and epithelium and skin barrier integrity, in order to establish the most adequate treatment for wound care after BASS grafting. Human keratinocytes (hKT) and dermal fibroblasts (hDF) were isolated from foreskin samples and integrated into hyaluronic acid-based BASS. The following antiseptics were applied every 48 h: ethanol (70%), chlorhexidine digluconate (1%), sodium hypochlorite (0.02%), povidone iodine (100 mg/mL), and polyhexanide (0.1%), during a follow-up of 16 days. Sodium hypochlorite was the only treatment that showed a high cell viability percentage throughout the evaluation time compared to other antiseptic treatments, as well as a similar cytokine secretion pattern as control BASS. No significant differences were found regarding epidermal barrier function. These findings point towards sodium hypochlorite being the least aggressive antiseptic treatment for BASS post-transplantation wound care.
PB MDPI AG
SN 2227-9059
YR 2022
FD 2022-06-17
LK http://hdl.handle.net/10668/20837
UL http://hdl.handle.net/10668/20837
LA en
NO We gratefully acknowledge financial support from the Ministry of Health andFamilies of the Andalusian Regional Government (PIGE-0242-2019) and from the Carlos III HealthInstitute (PI17/02083). The work of María I. Quiñones Vico was supported by a predoctoral fellowship(BOE22/10/2019) from the Ministry of Science, Innovation and Universities of Spain.
DS RISalud
RD Apr 18, 2025