%0 Journal Article %A Sanzo-Machuca, Ángela %A Monje Moreno, José Manuel %A Casado-Navarro, Rafael %A Karakuzu, Ozgur %A Guerrero-Gómez, David %A Fierro-González, Juan Carlos %A Swoboda, Peter %A Muñoz, Manuel J %A Garsin, Danielle A %A Pedrajas, José Rafael %A Barrios, Arantza %A Miranda-Vizuete, Antonio %T Redox-dependent and redox-independent functions of Caenorhabditis elegans thioredoxin 1. %D 2019 %U http://hdl.handle.net/10668/13795 %X Thioredoxins (TRX) are traditionally considered as enzymes catalyzing redox reactions. However, redox-independent functions of thioredoxins have been described in different organisms, although the underlying molecular mechanisms are yet unknown. We report here the characterization of the first generated endogenous redox-inactive thioredoxin in an animal model, the TRX-1 in the nematode Caenorhabditis elegans. We find that TRX-1 dually regulates the formation of an endurance larval stage (dauer) by interacting with the insulin pathway in a redox-independent manner and the cGMP pathway in a redox-dependent manner. Moreover, the requirement of TRX-1 for the extended longevity of worms with compromised insulin signalling or under calorie restriction relies on TRX-1 redox activity. In contrast, the nuclear translocation of the SKN-1 transcription factor and increased LIPS-6 protein levels in the intestine upon trx-1 deficiency are strictly redox-independent. Finally, we identify a novel function of C. elegans TRX-1 in male food-leaving behaviour that is redox-dependent. Taken together, our results position C. elegans as an ideal model to gain mechanistic insight into the redox-independent functions of metazoan thioredoxins, overcoming the limitations imposed by the embryonic lethal phenotypes of thioredoxin mutants in higher organisms. %K Caenorhabditis elegans %K Dauer %K Food-leaving %K Lips-6 %K Longevity %K Male %K Redox %K Skn-1 %K Thioredoxin %~