%0 Journal Article
%A Montañez, Maria I
%A Martin-Serrano, Angela
%A Mayorga, Cristobalina
%A Barrionuevo, Esther
%A Perez, Natalia
%A Romano, Antonino
%A Moreno, Esther
%A Ariza, Adriana
%A Perez-Inestrosa, Ezequiel
%A Torres, Maria J
%T Reply.
%D 2020
%U http://hdl.handle.net/10668/15491
%X We appreciate Baldo’s1 interest regarding our article,2 and his observations about both the precise cefaclor antigenic determinant structures and the uncertainly regarding structures attached in the solid phase. Concerning ‘‘solid-phase poly-L-Lysine-cefaclor,’’ no mild conditions were used to preserve the integrity of the cefaclor molecule in its conjugate fragmentation, resulting in an undefined mix of structures on the solid phase. Specifically, poly-L-Lysine-solid phases were treated with a cefaclor solution (10 mg/mL) in aqueous buffer at pH 10.2 (0.05 MNa2CO3/NaHCO3).
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