RT Journal Article
T1 Harmful R-loops are prevented via different cell cycle-specific mechanisms
A1 San Martin-Alonso, Marta
A1 Soler-Oliva, María E.
A1 García-Rubio, María
A1 García-Muse, Tatiana
A1 Aguilera, Andrés
K1 Cell Cycle
K1 DNA Damage
K1 DNA Helicases
K1 Genomic Instability
K1 Mutation
K1 Nuclear Proteins
K1 RNA, Fungal
K1 Ciclo Celular
K1 Daño del ADN
K1 ADN Helicasas
K1 Inestabilidad Genómica
K1 Mutación
K1 Proteínas Nucleares
K1 ARN de Hongos
AB Identifying how R-loops are generated is crucial to know how transcription compromises genome integrity. We show by genome-wide analysis of conditional yeast mutants that the THO transcription complex, prevents R-loop formation in G1 and S-phase, whereas the Sen1 DNA-RNA helicase prevents them only in S-phase. Interestingly, damage accumulates asymmetrically downstream of the replication fork in sen1 cells but symmetrically in the hpr1 THO mutant. Our results indicate that: R-loops form co-transcriptionally independently of DNA replication; that THO is a general and cell-cycle independent safeguard against R-loops, and that Sen1, in contrast to previously believed, is an S-phase-specific R-loop resolvase. These conclusions have important implications for the mechanism of R-loop formation and the role of other factors reported to affect on R-loop homeostasis.
PB Springer Nature
YR 2021
FD 2021-07-22
LK http://hdl.handle.net/10668/3470
UL http://hdl.handle.net/10668/3470
LA en
NO San Martin-Alonso M, Soler-Oliva ME, García-Rubio M, García-Muse T, Aguilera A. Harmful R-loops are prevented via different cell cycle-specific mechanisms. Nature Communications. 2021;12(1):4451.
DS RISalud
RD May 11, 2025