%0 Journal Article
%A San Martin-Alonso, Marta
%A Soler-Oliva, María E.
%A García-Rubio, María
%A García-Muse, Tatiana
%A Aguilera, Andrés
%T Harmful R-loops are prevented via different cell cycle-specific mechanisms
%D 2021
%U http://hdl.handle.net/10668/3470
%X Identifying how R-loops are generated is crucial to know how transcription compromises genome integrity. We show by genome-wide analysis of conditional yeast mutants that the THO transcription complex, prevents R-loop formation in G1 and S-phase, whereas the Sen1 DNA-RNA helicase prevents them only in S-phase. Interestingly, damage accumulates asymmetrically downstream of the replication fork in sen1 cells but symmetrically in the hpr1 THO mutant. Our results indicate that: R-loops form co-transcriptionally independently of DNA replication; that THO is a general and cell-cycle independent safeguard against R-loops, and that Sen1, in contrast to previously believed, is an S-phase-specific R-loop resolvase. These conclusions have important implications for the mechanism of R-loop formation and the role of other factors reported to affect on R-loop homeostasis.
%K Cell Cycle
%K DNA Damage
%K DNA Helicases
%K Genomic Instability
%K Mutation
%K Nuclear Proteins
%K RNA, Fungal
%K Ciclo Celular
%K Daño del ADN
%K ADN Helicasas
%K Inestabilidad Genómica
%K Mutación
%K Proteínas Nucleares
%K ARN de Hongos
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