RT Journal Article
T1 Hypoxia is associated with a lower expression of genes involved in lipogenesis in visceral adipose tissue.
A1 García-Fuentes, Eduardo
A1 Santiago-Fernández, Concepción
A1 Gutiérrez-Repiso, Carolina
A1 Mayas, María D
A1 Oliva-Olivera, Wilfredo
A1 Coín-Aragüez, Leticia
A1 Alcaide, Juan
A1 Ocaña-Wilhelmi, Luis
A1 Vendrell, Joan
A1 Tinahones, Francisco J
A1 Garrido-Sánchez, Lourdes
K1 Adipogénesis
K1 Tejido adiposo
K1 Índice de masa corporal
K1 Dieta
K1 Ayuno
K1 Factor 1 inducible por hipoxia
K1 Resistencia a la insulina
K1 Grasa intraabdominal
K1 Lipogénesis
K1 Ratones
K1 Humanos
K1 Obesidad mórbida
K1 PPAR gamma
K1 ARN mensajero
K1 Proteína 1 de unión a elementos reguladores de esteroles
AB BACKGROUND:A key role for HIF-1α in the promotion and maintenance of dietary obesity has been proposed. We analyzed the association between hypoxia and de novo lipogenesis in human adipose tissue.METHODS:We studied HIF-1α mRNA and protein expression in fasting status in visceral adipose tissue (VAT) from non-obese and morbidly obese subjects, and in VAT from wild-type and ob/ob C57BL6J mice in both fasting and feeding status. We also analyzed the effect of hypoxia on the VAT mRNA expression of genes involved in lipogenesis.RESULTS:HIF-1α was increased in VAT from morbidly obese subjects. In fasting status, C57BL6J ob/ob mice had a higher VAT HIF-1α mRNA expression than C57BL6J wild-type mice. In feeding status, VAT HIF-1α mRNA expression significantly increased in C57BL6J wild-type, but not in C57BL6J ob/ob mice. In humans, HIF-1α mRNA expression correlated positively with body mass index and insulin resistance. VAT HIF-1α mRNA expression correlated negatively with ACC1, PDHB and SIRT3 mRNA expression, and positively with PPAR-γ. VAT explants incubated in hypoxia showed reduced SIRT3 and increased PPAR-γ, SREBP-1c, ACLY, ACC1 and FASN mRNA expression.CONCLUSIONS:Morbidly obese subjects have a higher level of VAT HIF-1α. Postprandial status is associated with an increase in HIF-1α mRNA expression in C57BL6J wild-type mice. Hypoxia alters the mRNA expression of genes involved in de novo lipogenesis in human VAT.
PB Biomed Central
YR 2015
FD 2015-11-30
LK http://hdl.handle.net/10668/2449
UL http://hdl.handle.net/10668/2449
LA en
NO García-Fuentes E, Santiago-Fernández C, Gutiérrez-Repiso C, Mayas MD, Oliva-Olivera W, Coín-Aragüez L, et al. Hypoxia is associated with a lower expression of genes involved in lipogenesis in visceral adipose tissue. J Transl Med. 2015; 13:373
DS RISalud
RD Aug 23, 2025