%0 Journal Article
%A García-Fuentes, Eduardo
%A Santiago-Fernández, Concepción
%A Gutiérrez-Repiso, Carolina
%A Mayas, María D
%A Oliva-Olivera, Wilfredo
%A Coín-Aragüez, Leticia
%A Alcaide, Juan
%A Ocaña-Wilhelmi, Luis
%A Vendrell, Joan
%A Tinahones, Francisco J
%A Garrido-Sánchez, Lourdes
%T Hypoxia is associated with a lower expression of genes involved in lipogenesis in visceral adipose tissue.
%D 2015
%U http://hdl.handle.net/10668/2449
%X BACKGROUND:A key role for HIF-1α in the promotion and maintenance of dietary obesity has been proposed. We analyzed the association between hypoxia and de novo lipogenesis in human adipose tissue.METHODS:We studied HIF-1α mRNA and protein expression in fasting status in visceral adipose tissue (VAT) from non-obese and morbidly obese subjects, and in VAT from wild-type and ob/ob C57BL6J mice in both fasting and feeding status. We also analyzed the effect of hypoxia on the VAT mRNA expression of genes involved in lipogenesis.RESULTS:HIF-1α was increased in VAT from morbidly obese subjects. In fasting status, C57BL6J ob/ob mice had a higher VAT HIF-1α mRNA expression than C57BL6J wild-type mice. In feeding status, VAT HIF-1α mRNA expression significantly increased in C57BL6J wild-type, but not in C57BL6J ob/ob mice. In humans, HIF-1α mRNA expression correlated positively with body mass index and insulin resistance. VAT HIF-1α mRNA expression correlated negatively with ACC1, PDHB and SIRT3 mRNA expression, and positively with PPAR-γ. VAT explants incubated in hypoxia showed reduced SIRT3 and increased PPAR-γ, SREBP-1c, ACLY, ACC1 and FASN mRNA expression.CONCLUSIONS:Morbidly obese subjects have a higher level of VAT HIF-1α. Postprandial status is associated with an increase in HIF-1α mRNA expression in C57BL6J wild-type mice. Hypoxia alters the mRNA expression of genes involved in de novo lipogenesis in human VAT.
%K Adipogénesis
%K Tejido adiposo
%K Índice de masa corporal
%K Dieta
%K Ayuno
%K Factor 1 inducible por hipoxia
%K Resistencia a la insulina
%K Grasa intraabdominal
%K Lipogénesis
%K Ratones
%K Humanos
%K Obesidad mórbida
%K PPAR gamma
%K ARN mensajero
%K Proteína 1 de unión a elementos reguladores de esteroles
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