RT Generic
T1 When DNA gets in the way: A cautionary note for DNA contamination in extracellular RNA-seq studies.
A1 Verwilt, Jasper
A1 Trypsteen, Wim
A1 Van-Paemel, Ruben
A1 De-Preter, Katleen
A1 Giraldez, Maria D
A1 Mestdagh, Pieter
A1 Vandesompele, Jo
K1 Extracellular RNA analysis
K1 Sequencing artifacts
K1 Contaminant detection
K1 Data reproducibility
K1 Liquid biopsy methods
K1 Low-input sequencing
AB With great interest, we read the paper by Zhou et al. (1) describing a methodology that enables extracellular RNA sequencing (exRNA-seq) from extremely low input (Small Input Liquid Volume Extracellular RNA Sequencing [SILVER-seq]). We were intrigued by the high number of detected genes compared to our previous studies (2, 3) and noticed low reproducibility. We hypothesized that these observations could originate from substantial DNA contamination. Therefore, we reanalyzed the SILVER-seq data (4) to determine the extent of DNA signal in the sequencing reads.
PB National Academy of Sciences
YR 2020
FD 2020-08-11
LK http://hdl.handle.net/10668/16096
UL http://hdl.handle.net/10668/16096
LA en
NO Verwilt J, Trypsteen W, Van Paemel R, De Preter K, Giraldez MD, Mestdagh P, et al. When DNA gets in the way: A cautionary note for DNA contamination in extracellular RNA-seq studies. Proc Natl Acad Sci U S A. 2020 Aug 11;117(32):18934-18936.
DS RISalud
RD Oct 22, 2025