RT Journal Article
T1 Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts.
A1 Islam, Mohammad Mirazul
A1 Sharifi, Roholah
A1 Mamodaly, Shamina
A1 Islam, Rakibul
A1 Nahra, Daniel
A1 Abusamra, Dina B
A1 Hui, Pui Chuen
A1 Adibnia, Yashar
A1 Goulamaly, Mehdi
A1 Paschalis, Eleftherios I
A1 Cruzat, Andrea
A1 Kong, Jing
A1 Nilsson, Per H
A1 Argüeso, Pablo
A1 Mollnes, Tom Eirik
A1 Chodosh, James
A1 Dohlman, Claes H
A1 Gonzalez-Andrades, Miguel
K1 Acellular porcine cornea
K1 Corneal transplant
K1 Decellularization
K1 Gamma irradiation sterilization
K1 Recellularization
AB To address the shortcomings associated with corneal transplants, substantial efforts have been focused on developing new modalities such as xenotransplantion. Xenogeneic corneas are anatomically and biomechanically similar to the human cornea, yet their applications require prior decellularization to remove the antigenic components to avoid rejection. In the context of bringing decellularized corneas into clinical use, sterilization is a crucial step that determines the success of the transplantation. Well-standardized sterilization methods, such as gamma irradiation (GI), have been applied to decellularized porcine corneas (DPC) to avoid graft-associated infections in human recipients. However, little is known about the effect of GI on decellularized corneal xenografts. Here, we evaluated the radiation effect on the ultrastructure, optical, mechanical and biological properties of DPC. Transmission electron microscopy revealed that gamma irradiated decellularized porcine cornea (G-DPC) preserved its structural integrity. Moreover, the radiation did not reduce the optical properties of the tissue. Neither DPC nor G-DPC led to further activation of complement system compared to native porcine cornea when exposed to plasma. Although, DPC were mechanically comparable to the native tissue, GI increased the mechanical strength, tissue hydrophobicity and resistance to enzymatic degradation. Despite these changes, human corneal epithelial, stromal, endothelial and hybrid neuroblastoma cells grew and differentiated on DPC and G-DPC. Thus, GI may achieve effective tissue sterilization without affecting critical properties that are essential for corneal transplant survival.
PB Elsevier
YR 2019
FD 2019-07-05
LK http://hdl.handle.net/10668/14222
UL http://hdl.handle.net/10668/14222
LA en
NO Islam MM, Sharifi R, Mamodaly S, Islam R, Nahra D, Abusamra DB, et al. Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts. Acta Biomater. 2019 Sep 15;96:330-344
NO This paper was supported by Boston-KPro research fund: Boston, MA, USA; and NIH National Eye Institute: Bethesda, MD, USA; Core Grant 30EY003790, and an unrestricted grant to the Department of Ophthalmology, Harvard Medical School, by Research to Prevent Blindness, NY, NY. Financial support was also obtained from The Odd Fellow Foundation: Oslo, Norway; and The Simon Fougner Hartmann Family: Dragør, Denmark Fund. PCH was supported by the Croucher Fellowship. MG and JK acknowledge the support of HK ITC grant reference: ITS/195/14FP. Access to the PS-OCT instrumentation and processing tools was provided by the Center forBiomedical OCT Research and Translation, which is supported by the Nationals Institutes of Health (grant P41EB-015903). The authors gratefully acknowledge support from the Department of Biological Engineering ofMIT for gamma irradiation and Oscar Morales (Schepens Eye Research Institute) for assisting with the OCT ex vivo work. We also appreciate the aid of Bianai Fan and Philip Seifert (Schepens Eye Research Institute) in the lightand electron microscopy studies, respectively.
DS RISalud
RD Apr 6, 2025