RT Journal Article
T1 Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation.
A1 Carrillo-Jimenez, Alejandro
A1 Puigdellívol, Mar
A1 Vilalta, Anna
A1 Venero, Jose Luis
A1 Brown, Guy Charles
A1 StGeorge-Hyslop, Peter
A1 Burguillos, Miguel Angel
K1 Alzheimer’s disease
K1 CD33
K1 CGC
K1 TREM2
K1 microglia
K1 siRNA
K1 transfection
AB Microglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer's disease (AD). The use of primary microglial cell cultures has proved to be a valuable tool to study microglial biology under various conditions. However, more advanced transfection methodologies for primary cultured microglia are still needed, as current methodologies provide low transfection efficiency and induce cell death and/or inflammatory activation of the microglia. Here, we describe an easy, and effective method based on the Glial-Mag method (OZ Biosciences) using magnetic nanoparticles and a magnet to successfully transfect primary microglia cells with different small interfering RNAs (siRNAs). This method does not require specialist facilities or specific training and does not induce cell toxicity or inflammatory activation. We demonstrate that this protocol successfully decreases the expression of two key genes associated with AD, the triggering receptor expressed in myeloid cells 2 (TREM2) and CD33, in primary microglia cell cultures.
SN 1662-5102
YR 2018
FD 2018-09-21
LK https://hdl.handle.net/10668/27381
UL https://hdl.handle.net/10668/27381
LA en
DS RISalud
RD Apr 6, 2025