RT Journal Article
T1 Alterations of specific biomarkers of metabolic pathways in vascular tree from patients with Type 2 diabetes.
A1 Bernal-López, María Rosa
A1 Llorente-Cortés, Vicenta
A1 Gómez-Carrillo, Víctor
A1 López-Carmona, Dolores
A1 Calleja, Fernando
A1 Gómez-Huelgas, Ricardo
A1 Badimón, Lina
A1 Tinahones, Francisco J
K1 Aterosclerosis
K1 Marcadores Biológicos
K1 Isquemia Miocárdica
K1 Inflamación
K1 Diabetes Mellitus
K1 Arteriopatías Oclusivas
K1 Inhibidor p21 de las Quinasas Dependientes de la Ciclina
K1 Ciclooxigenasa 2
K1 Metaloproteinasa 2 de la Matriz
K1 Metaloproteinasa 9 de la Matriz
K1 Hemoglobina A Glucosilada
K1 Inhibidores Tisulares de Metaloproteinasas
K1 Receptor Toll-Like 2
K1 Diabetes Mellitus tipo 2
K1 Metabolismo de los Lípidos
K1 Reacción en Cadena en Tiempo Real de la Polimerasa
K1 Humanos
AB The aims of this study were to check whether different biomarkers of inflammatory, apoptotic, immunological or lipid pathways had altered their expression in the occluded popliteal artery (OPA) compared with the internal mammary artery (IMA) and femoral vein (FV) and to examine whether glycemic control influenced the expression of these genes. The study included 20 patients with advanced atherosclerosis and type 2 diabetes mellitus, 15 of whom had peripheral arterial occlusive disease (PAOD), from whom samples of OPA and FV were collected. PAOD patients were classified based on their HbA1c as well (HbA1c ≤ 6.5) or poorly (HbA1c > 6.5) controlled patients. Controls for arteries without atherosclerosis comprised 5 IMA from patients with ischemic cardiomyopathy (ICM). mRNA, protein expression and histological studies were analyzed in IMA, OPA and FV. After analyzing 46 genes, OPA showed higher expression levels than IMA or FV for genes involved in thrombosis (F3), apoptosis (MMP2, MMP9, TIMP1 and TIM3), lipid metabolism (LRP1 and NDUFA), immune response (TLR2) and monocytes adhesion (CD83). Remarkably, MMP-9 expression was lower in OPA from well-controlled patients. In FV from diabetic patients with HbA1c ≤6.5, gene expression levels of BCL2, CDKN1A, COX2, NDUFA and SREBP2 were higher than in FV from those with HbA1c >6.5. The atherosclerotic process in OPA from diabetic patients was associated with high expression levels of inflammatory, lipid metabolism and apoptotic biomarkers. The degree of glycemic control was associated with gene expression markers of apoptosis, lipid metabolism and antioxidants in FV. However, the effect of glycemic control on pro-atherosclerotic gene expression was very low in arteries with established atherosclerosis.
PB BioMed Central
YR 2012
FD 2012-07-24
LK http://hdl.handle.net/10668/762
UL http://hdl.handle.net/10668/762
LA en
NO Bermal-López MR, Llorente Cortés V, Gómez Carrillo V, López Carmona D, Calleja F, Gómez Huelgas R, et al. Alterations of specific biomarkers of metabolic pathways in vascular tree from patients with Type 2 diabetes. Cardiovasc Diabetol; 11:86
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 12, 2025