RT Journal Article
T1 Analysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter baumannii.
A1 Cayô, Rodrigo
A1 Rodríguez, María-Cruz
A1 Espinal, Paula
A1 Fernández-Cuenca, Felipe
A1 Ocampo-Sosa, Alain A
A1 Pascual, Alvaro
A1 Ayala, Juan A
A1 Vila, Jordi
A1 Martínez-Martínez, Luis
K1 Secuencia de Aminoácidos
K1 Hospitales Escuela
K1 Pruebas de Sensibilidad Microbiana
K1 Proteínas de Unión a las Penicilinas
K1 Análisis de Secuencia de ADN
K1 Resistencia beta-Lactámica
AB There is limited information on the role of penicillin-binding proteins (PBPs) in the resistance of Acinetobacter baumannii to β-lactams. This study presents an analysis of the allelic variations of PBP genes in A. baumannii isolates. Twenty-six A. baumannii clinical isolates (susceptible or resistant to carbapenems) from three teaching hospitals in Spain were included. The antimicrobial susceptibility profile, clonal pattern, and genomic species identification were also evaluated. Based on the six complete genomes of A. baumannii, the PBP genes were identified, and primers were designed for each gene. The nucleotide sequences of the genes identified that encode PBPs and the corresponding amino acid sequences were compared with those of ATCC 17978. Seven PBP genes and one monofunctional transglycosylase (MGT) gene were identified in the six genomes, encoding (i) four high-molecular-mass proteins (two of class A, PBP1a [ponA] and PBP1b [mrcB], and two of class B, PBP2 [pbpA or mrdA] and PBP3 [ftsI]), (ii) three low-molecular-mass proteins (two of type 5, PBP5/6 [dacC] and PBP6b [dacD], and one of type 7 (PBP7/8 [pbpG]), and (iii) a monofunctional enzyme (MtgA [mtgA]). Hot spot mutation regions were observed, although most of the allelic changes found translated into silent mutations. The amino acid consensus sequences corresponding to the PBP genes in the genomes and the clinical isolates were highly conserved. The changes found in amino acid sequences were associated with concrete clonal patterns but were not directly related to susceptibility or resistance to β-lactams. An insertion sequence disrupting the gene encoding PBP6b was identified in an endemic carbapenem-resistant clone in one of the participant hospitals.
PB American Society for Microbiology
SN 0066-4804
YR 2011
FD 2011-12
LK http://hdl.handle.net/10668/2055
UL http://hdl.handle.net/10668/2055
LA en
NO Cayô R, Rodríguez MC, Espinal P, Fernández-Cuenca F, Ocampo-Sosa AA, Pascual A, et al. Analysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter baumannii. Antimicrob. Agents Chemother. 2011; 55(12):5907-13
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 9, 2025