%0 Generic
%A Bhatia, Vaibhav
%A Herrera-Moyano, Emilia
%A Aguilera, Andres
%A Gomez-Gonzalez, Belen
%T The Role of Replication-Associated Repair Factors on R-Loops
%D 2017
%@ 2073-4425
%U http://hdl.handle.net/10668/19261
%X The nascent RNA can reinvade the DNA double helix to form a structure termed the R-loop, where a single-stranded DNA (ssDNA) is accompanied by a DNA-RNA hybrid. Unresolved R-loops can impede transcription and replication processes and lead to genomic instability by a mechanism still not fully understood. In this sense, a connection between R-loops and certain chromatin markers has been reported that might play a key role in R-loop homeostasis and genome instability. To counteract the potential harmful effect of R-loops, different conserved messenger ribonucleoprotein (mRNP) biogenesis and nuclear export factors prevent R-loop formation, while ubiquitously-expressed specific ribonucleases and DNA-RNA helicases resolve DNA-RNA hybrids. However, the molecular events associated with R-loop sensing and processing are not yet known. Given that R-loops hinder replication progression, it is plausible that some DNA replication-associated factors contribute to dissolve R-loops or prevent R-loop mediated genome instability. In support of this, R-loops accumulate in cells depleted of the BRCA1, BRCA2 or the Fanconi anemia (FA) DNA repair factors, indicating that they play an active role in R-loop dissolution. In light of these results, we review our current view of the role of replication-associated DNA repair pathways in preventing the harmful consequences of R-loops.
%K genetic instability
%K DNA-RNA hybrids
%K Fanconi anemia
%K BRCA
%K replication stress
%K cancer
%K Mediated genome instability
%K Dna-rna hybrids
%K Cancer susceptibility protein
%K Transcriptional pause sites
%K Fanconi anemia/brca pathway
%K Common fragile sites
%K Cross-link repair
%K Saccharomyces-cerevisiae
%K Fork progression
%K Rna/dna hybrids
%~