RT Journal Article T1 Designing Single-Molecule Magnets as Drugs with Dual Anti-Inflammatory and Anti-Diabetic Effects. A1 Navas, Arturo A1 Jannus, Fatin A1 Fernández, Belén A1 Cepeda, Javier A1 Medina O'Donnell, Marta A1 Díaz-Ruiz, Luis A1 Sánchez-González, Cristina A1 Llopis, Juan A1 Seco, José M A1 Rufino-Palomares, E A1 Lupiáñez, José Antonio A1 Gómez-Ruiz, Santiago A1 Quiles, José Luis A1 Battino, Maurizio A1 Choquesillo-Lazarte, Duane A1 Ruiz-Muelle, Ana Belén A1 Fernández, Ignacio A1 Reyes-Zurita, Fernando A1 Rodríguez-Diéguez, Antonio K1 bumetadine K1 cobalt K1 coordination compound K1 diabetes K1 indomethacin K1 inflammatory K1 single-ion magnet AB We have designed and synthesized two novel cobalt coordination compounds using bumetanide (bum) and indomethacin (ind) therapeutic agents. The anti-inflammatory effects of cobalt metal complexes with ind and bum were assayed in lipopolysaccharide stimulated RAW 264.7 macrophages by inhibition of nitric oxide production. Firstly, we determined the cytotoxicity and the anti-inflammatory potential of the cobalt compounds and ind and bum ligands in RAW 264.7 cells. Indomethacin-based metal complex was able to inhibit the NO production up to 35% in a concentration-dependent manner without showing cytotoxicity, showing around 6-37 times more effective than indomethacin. Cell cycle analysis showed that the inhibition of NO production was accompanied by a reversion of the differentiation processes in LPS-stimulated RAW 264.7 cells, due to a decreased of cell percentage in G0/G1 phase, with the corresponding increase in the number of cells in S phase. These two materials have mononuclear structures and show slow relaxation of magnetization. Moreover, both compounds show anti-diabetic activity with low in vitro cell toxicities. The formation of metal complexes with bioactive ligands is a new and promising strategy to find new compounds with high and enhanced biochemical properties and promises to be a field of great interest. YR 2020 FD 2020-04-29 LK http://hdl.handle.net/10668/15497 UL http://hdl.handle.net/10668/15497 LA en DS RISalud RD Apr 8, 2025