RT Journal Article
T1 How Meaningful Are Minor Details in the Generation of Nanomodified Electrochemical Enzyme Biosensors? Exploring the Scenario with Sinusoidal Approaches
A1 Rahman, Md Towhidur
A1 Lopez-Iglesias, David
A1 Sierra-Padilla, Alfonso
A1 Garcia-Guzman, Juan Jose
A1 Cubillana-Aguilera, Laura M.
A1 Bellido-Milla, Dolores
A1 Palacios-Santander, Jose Maria
K1 Sonogel-carbon (bio)sensors
K1 Gold nanoparticles
K1 Carbon nanotubes
K1 Tyrosinase
K1 Sinusoidal methods
K1 3,4-ethylenedioxythiophene
K1 Dopamine
K1 Screen-printed electrodes
K1 Reduced graphene oxide
K1 Amperometric biosensors
K1 Dopamine
K1 Nanoparticles
K1 Glucose
K1 Cholesterol
K1 Design
K1 Film
K1 Nanocomposite
AB In this work, a screening of Sonogel-Carbon (SNGC) electrodes modified with nanomaterials (carbon nanotubes and gold nanoparticles) and the study of their effect on the electrochemical performance of sinusoidal voltage (SV) and current (SC)-based biosensors are reported. Surface modification was achieved by drop-casting and electrodeposition methodologies. Within the strategies used, SV and SC, recently exploited procedures, were used to electrodeposit simultaneously a poly 3,4-ethylenedioxythiophene (PEDOT)-tyrosinase layer and the corresponding nanostructured material. Dopamine was selected as a benchmark analyte to evaluate the analytical performance of the different (bio)sensors obtained in terms of relevant figures of merit, such as sensitivity, limits of detection and quantitation, and accuracy, among others. A discussion about the pros and cons between the type of modification and the methods employed is also presented. Briefly, SC based sensors offered excellent quality analytical parameters and lower dispersion of the results. They were employed for more specific electrochemical studies, including interferences assays and the determination of DA in real samples, obtaining good recoveries (101-110.6%). The biosensor modified with gold nanoparticles (AuNPs) (drop-casting method) and SC-electrodeposited showed the best figures of merit: R-2 = 0.999; sensitivity = -4.92 x 10(-9) A.mu M-1; RSDsensitivity = 1.60%; LOD = 5.56 mu M; RSDLOD = 6.10%; and LOQ = 18.53 mu M.
PB MDPI
YR 2022
FD 2022-08-07
LK http://hdl.handle.net/10668/20942
UL http://hdl.handle.net/10668/20942
LA en
NO Rahman MdT, López-Iglesias D, Sierra-Padilla A, García-Guzmán JJ, Cubillana-Aguilera LM, Bellido-Milla D, et al. How Meaningful Are Minor Details in the Generation of Nanomodified Electrochemical Enzyme Biosensors? Exploring the Scenario with Sinusoidal Approaches. Chemosensors [Internet]. 7 de agosto de 2022;10(8):316
NO Authors thank Electron Microscopy Division from Servicios Centrales de Investigación Científica y Tecnológica of University of Cadiz (SC-ICYT UCA) for their technical assistance during SEM measurements.
DS RISalud
RD Apr 14, 2025