TY - JOUR AU - Nakano, Miyako AU - Sabido-Bozo, Susana AU - Okazaki, Kouta AU - Aguilera-Romero, Auxiliadora AU - Rodriguez-Gallardo, Sofia AU - Cortes-Gomez, Alejandro AU - Lopez, Sergio AU - Ikeda, Atsuko AU - Funato, Kouichi AU - Muñiz, Manuel PY - 2021 DO - 10.1371/journal.pone.0257435 UR - http://hdl.handle.net/10668/4410 T2 - PLoS One AB - Glycosylphosphatidylinositol (GPI) anchoring of proteins is an essential post-translational modification in all eukaryotes that occurs at the endoplasmic reticulum (ER) and serves to deliver GPI-anchored proteins (GPI-APs) to the cell surface where... LA - en PB - Public Library of Science KW - Glycosylphosphatidylinositol KW - Trypsin KW - Endoplasmic reticulum KW - Mass spectrometry KW - Electrophoresis KW - Digestion KW - Peptides KW - Glicosilfosfatidilinositoles KW - Tripsina KW - Retículo endoplásmico KW - Espectrometría de masas KW - Electroforesis KW - Digestión KW - Péptidos KW - Medical Subject Headings::Anatomy::Cells::Cellular Structures::Intracellular Space::Cytoplasm::Cytoplasmic Structures::Organelles::Endoplasmic Reticulum KW - Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::GPI-Linked Proteins KW - Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides KW - Medical Subject Headings::Organisms::Eukaryota::Fungi::Ascomycota::Saccharomycetales::Saccharomyces::Saccharomyces cerevisiae KW - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Tandem Mass Spectrometry KW - Medical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycoconjugates::Glycolipids::Glycosylphosphatidylinositols KW - Medical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Endopeptidases::Serine Endopeptidases::Trypsin KW - Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Biosynthetic Pathways KW - Medical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Protein Modification, Translational::Protein Processing, Post-Translational KW - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry KW - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis KW - Medical Subject Headings::Phenomena and Processes::Digestive System and Oral Physiological Phenomena::Digestive System Physiological Phenomena::Digestive System Processes::Digestion TI - Structural analysis of the GPI glycan TY - research article ER -