RT Journal Article
T1 Pep19 Has a Positive Effect on Insulin Sensitivity and Ameliorates Both Hepatic and Adipose Tissue Phenotype of Diet-Induced Obese Mice.
A1 Silvério, Renata
A1 Barth, Robson
A1 Heimann, Andrea S
A1 Reckziegel, Patrícia
A1 Dos Santos, Gustavo J
A1 Romero-Zerbo, Silvana Y
A1 Bermudez-Silva, Francisco J
A1 Rafacho, Alex
A1 Ferro, Emer S
K1 bioactive peptides
K1 intracellular peptides
K1 metabolism
K1 obesity
K1 orally active peptides
K1 overweight
AB Peptide DIIADDEPLT (Pep19) has been previously suggested to improve metabolic parameters, without adverse central nervous system effects, in a murine model of diet-induced obesity. Here, we aimed to further evaluate whether Pep19 oral administration has anti-obesogenic effects, in a well-established high-fat diet-induced obesity model. Male Swiss mice, fed either a standard diet (SD) or high-fat diet (HFD), were orally administrated for 30 consecutive days, once a day, with saline vehicle or Pep19 (1 mg/kg). Next, several metabolic, morphological, and behavioral parameters were evaluated. Oral administration of Pep19 attenuated HFD body-weight gain, reduced in approximately 40% the absolute mass of the endocrine pancreas, and improved the relationship between circulating insulin and peripheral insulin sensitivity. Pep19 treatment of HFD-fed mice attenuated liver inflammation, hepatic fat distribution and accumulation, and lowered plasma alanine aminotransferase activity. The inguinal fat depot from the SD group treated with Pep19 showed multilocular brown-fat-like cells and increased mRNA expression of uncoupling protein 1 (UCP1), suggesting browning on inguinal white adipose cells. Morphological analysis of brown adipose tissue (BAT) from HFD mice showed the presence of larger white-like unilocular cells, compared to BAT from SD, Pep19-treated SD or HFD mice. Pep19 treatment produced no alterations in mice behavior. Oral administration of Pep19 ameliorates some metabolic traits altered by diet-induced obesity in a Swiss mice model.
PB MDPI
YR 2022
FD 2022-04-01
LK http://hdl.handle.net/10668/21149
UL http://hdl.handle.net/10668/21149
LA en
NO Silvério R, Barth R, Heimann AS, Reckziegel P, Dos Santos GJ, Romero-Zerbo SY, et al. Pep19 Has a Positive Effect on Insulin Sensitivity and Ameliorates Both Hepatic and Adipose Tissue Phenotype of Diet-Induced Obese Mice. Int J Mol Sci. 2022 Apr 7;23(8):4082
DS RISalud
RD Apr 10, 2025