RT Journal Article
T1 Changes in High-Density Lipoproteins Related to Outcomes in Patients with Acute Stroke
A1 Varela, Lourdes M.
A1 Meseguer, Elena
A1 Lapergue, Bertrand
A1 Couret, David
A1 Amarenco, Pierre
A1 Meilhac, Olivier
K1 HDL
K1 Stroke
K1 Functional outcome
K1 PON1
K1 Blood-brain barrier (BBB) dysfunction
K1 Low-density lipoproteins (LDL) oxidation
K1 Paraoxonase-1
K1 High-density lipoprotein
K1 Accidente cerebrovascular
K1 Lipoproteínas LDL
K1 Arildialquilfosfatasa
K1 Lipoproteínas HDL
AB Modifications in high-density lipoprotein (HDL) particle sizes and HDL-binding proteins have been reported in stroke patients. We evaluated whether the lipoprotein profile, HDL composition and functionality were altered in stroke patients according to their clinical outcome using the modified Rankin Score at 3 months. Plasma samples were obtained from stroke patients treated with intravenous thrombolysis. Levels of cardiovascular and inflammatory markers in plasma were measured using the Human CVD Panel 1 (Milliplex® MAP). Lipoprotein subfractions from plasma were quantified by non-denaturing acrylamide gel electrophoresis, using the Lipoprint®-System (Quantimetrix®), and HDLs were isolated by ultracentrifugation. Relative amounts of paraoxonase-1 (PON1) and alpha-1 anti-trypsin (AAT) in the isolated HDLs were determined by Western blot. HDL anti-inflammatory function was evaluated in human blood-brain barrier endothelial cells stimulated with 100 ng/mL TNFα, and HDL antioxidant function was evaluated via their capacity to limit copper-induced low-density lipoprotein oxidation. Stroke patients with unfavorable outcomes had a lower proportion of small-sized HDLs and increased plasma levels of E-selectin (SELE) and the intercellular adhesion molecule 1 (ICAM1). HDLs from patients with unfavorable outcomes had lower levels of PON1 and displayed a blunted capacity to reduce the expression of SELE, interleukin 8 (IL8) and the monocyte chemoattractant protein-1 (MCP1) mRNA induced by TNFα in endothelial cells. These HDLs also had a reduced antioxidant capacity relative to HDLs from healthy donors. In conclusion, an increased ratio of large/small HDLs with impaired anti-inflammatory and antioxidant capacities was associated with unfavorable outcomes in stroke patients. Alteration of HDL functionality was mainly associated with a low amount of PON1 and high amount of AAT.
PB MDPI
YR 2020
FD 2020-07-17
LK http://hdl.handle.net/10668/4386
UL http://hdl.handle.net/10668/4386
LA en
NO Varela LM, Meseguer E, Lapergue B, Couret D, Amarenco P, Meilhac O. Changes in High-Density Lipoproteins Related to Outcomes in Patients with Acute Stroke. J Clin Med. 2020 Jul 17;9(7):2269
DS RISalud
RD Apr 19, 2025