RT Journal Article
T1 Phylogeny, resistome and mobile genetic elements of emergent OXA-48 and OXA-245 Klebsiella pneumoniae clones circulating in Spain.
A1 Perez-Vazquez, Maria
A1 Oteo, Jesus
A1 Garcia-Cobos, Silvia
A1 Aracil, Belen
A1 Harris, Simon R
A1 Ortega, Adriana
A1 Fontanals, Dionisia
A1 Hernandez, Juan Manuel
A1 Solís, Sonia
A1 Campos, Jose
A1 Dougan, Gordon
A1 Kingsley, Robert A
K1 Bacterial Proteins
K1 Cross Infection
K1 DNA, Bacterial
K1 Disease Outbreaks
AB The global emergence of OXA-48-producing Klebsiella pneumoniae clones is a significant threat to public health. We used WGS and phylogenetic analysis of Spanish isolates to investigate the population structure of blaOXA-48-like-expressing K. pneumoniae ST11 and ST405 and to determine the distribution of resistance genes and plasmids encoding blaOXA-48-like carbapenemases. SNPs identified in whole-genome sequences were used to reconstruct phylogenetic trees, identify resistance determinants and de novo assemble the genomes of 105 blaOXA-48-like-expressing K. pneumoniae isolates. Genome variation was generally lower in outbreak-associated isolates compared with those associated with sporadic infections. The relatively limited variation observed within the outbreak-associated isolates was on average 7-10 SNPs per outbreak. Of 24 isolates from suspected sporadic infections, 7 were very closely related to isolates causing hospital outbreaks and 17 were more diverse and therefore probably true sporadic cases. On average, 14 resistance genes were identified per isolate. The 17 ST405 isolates from sporadic cases of infection had four distinct resistance gene profiles, while the resistance gene profile differed in all ST11 isolates from sporadic cases. Sequence analysis of 94 IncL/M plasmids carrying blaOXA-48-like genes revealed an average of two SNP differences, indicating a conserved plasmid clade. Whole-genome sequence analysis enabled the discrimination of outbreak and sporadic isolates. Significant inter-regional spread within Spain of highly related isolates was evident for both ST11 and ST405 K. pneumoniae. IncL/M plasmids carrying blaOXA-48-like carbapenemase genes were highly conserved geographically and across the outbreaks, sporadic cases and clones.
PB Oxford University Press
YR 2016
FD 2016-01-13
LK http://hdl.handle.net/10668/9736
UL http://hdl.handle.net/10668/9736
LA en
NO Pérez-Vázquez M, Oteo J, García-Cobos S, Aracil B, Harris SR, Ortega A, et al. Phylogeny, resistome and mobile genetic elements of emergent OXA-48 and OXA-245 Klebsiella pneumoniae clones circulating in Spain. J Antimicrob Chemother. 2016 Apr;71(4):887-96
NO This study was supportedbytheMinisterio de Economía y Competitividad, Instituto de Salud Carlos III (BA12/00022) and Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economıía y Competitividad, the Spanish Network for Research in Infectious Diseases (REIPI RD12/0015), Fondo de Investigación Sanitaria (grant number PI12/01242) and Wellcome Trust UK (grant no. 098051).
DS RISalud
RD Apr 4, 2025