RT Journal Article
T1 Urinary Tract Conditions Affect Fosfomycin Activity against Escherichia coli Strains Harboring Chromosomal Mutations Involved in Fosfomycin Uptake
A1 Martin-Gutierrez, G.
A1 Docobo-Perez, F.
A1 Rodriguez-Beltran, J.
A1 Rodriguez-Martinez, J. M.
A1 Aznar, J.
A1 Pascual, A.
A1 Blazquez, J.
K1 fosfomycin activity
K1 fosfomycin resistance
K1 chromosomal mutations
K1 UTI
K1 Escherichia coli
K1 urinary tract infection
K1 Resistant klebsiella-pneumoniae
K1 Dose oral treatment
K1 Intravenous fosfomycin
K1 Bacterial-growth
K1 Infections
K1 Impact
K1 Tissue
K1 Phosphonomycin
K1 Susceptibility
K1 Epidemiology
AB The steps by which Escherichia coli strains harboring mutations related to fosfomycin (FOS) resistance arise and spread during urinary tract infections (UTIs) are far from being understood. The aim of this study was to evaluate the effects of urine, pH, and anaerobiosis on FOS activity against a set of isogenic strains carrying the most prevalent chromosomal mutations conferring FOS resistance (Delta uhpT,Delta glpT,Delta cyaA, and Delta ptsI), either singly or in combination. We also studied fosfomycin-resistant E. coli clinical isolates from patients with UTI. Our results demonstrate that urinary tract physiological conditions might have a profound impact on FOS activity against strains with chromosomal FOS resistance mutations. Specifically, acidic pH values and anaerobiosis convert most of the strains categorized as resistant to fosfomycin according to the international guidelines to a susceptible status. Therefore, urinary pH values may have practical interest in the management of UTIs. Finally, our results, together with the high fitness cost associated with FOS resistance mutations, might explain the low prevalence of fosfomycin-resistant E. coli variants in UTIs.
PB Amer soc microbiology
SN 0066-4804
YR 2018
FD 2018-01-01
LK http://hdl.handle.net/10668/19005
UL http://hdl.handle.net/10668/19005
LA en
DS RISalud
RD Apr 19, 2025