%0 Journal Article
%A Clemente-Postigo, Mercedes
%A Queipo-Ortuño, María Isabel
%A Fernández-García, Diego José
%A Gómez-Huelgas, Ricardo
%A Tinahones, Francisco J
%A Cardona Díaz, Fernando
%T Adipose tissue gene expression of factors related to lipid processing in obesity.
%D 2011
%U http://hdl.handle.net/10668/694
%X BACKGROUNDAdipose tissue lipid storage and processing capacity can be a key factor for obesity-related metabolic disorders such as insulin resistance and diabetes. Lipid uptake is the first step to adipose tissue lipid storage. The aim of this study was to analyze the gene expression of factors involved in lipid uptake and processing in subcutaneous (SAT) and visceral (VAT) adipose tissue according to body mass index (BMI) and the degree of insulin resistance (IR).METHODS AND PRINCIPAL FINDINGSVLDL receptor (VLDLR), lipoprotein lipase (LPL), acylation stimulating protein (ASP), LDL receptor-related protein 1 (LRP1) and fatty acid binding protein 4 (FABP4) gene expression was measured in VAT and SAT from 28 morbidly obese patients with Type 2 Diabetes Mellitus (T2DM) or high IR, 10 morbidly obese patients with low IR, 10 obese patients with low IR and 12 lean healthy controls. LPL, FABP4, LRP1 and ASP expression in VAT was higher in lean controls. In SAT, LPL and FABP4 expression were also higher in lean controls. BMI, plasma insulin levels and HOMA-IR correlated negatively with LPL expression in both VAT and SAT as well as with FABP4 expression in VAT. FABP4 gene expression in SAT correlated inversely with BMI and HOMA-IR. However, multiple regression analysis showed that BMI was the main variable contributing to LPL and FABP4 gene expression in both VAT and SAT.CONCLUSIONSMorbidly obese patients have a lower gene expression of factors related with lipid uptake and processing in comparison with healthy lean persons.
%K LRP1 protein, human
%K acylation stimulating protein, human
%K Proteínas de Unión a Ácidos Grasos
%K Índice de masa corporal
%K Diabetes Mellitus Tipo 2
%K FABP4 protein, human
%K Expresión genética
%K Resistencia a la insulina
%K Péptidos y Proteínas de Señalización Intercelular
%K Grasa intraabdominal
%K Metabolismo de los Lípidos
%K Lipoproteína Lipasa
%K Proteina 1 Relacionada con Receptor de Lipoproteína de Baja Densidad
%K Obesidad mórbida
%K Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
%K Grasa subcutánea
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