RT Journal Article
T1 Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers.
A1 Altmäe, Signe
A1 Koel, Mariann
A1 Võsa, Urmo
A1 Adler, Priit
A1 Suhorutšenko, Marina
A1 Laisk-Podar, Triin
A1 Kukushkina, Viktorija
A1 Saare, Merli
A1 Velthut-Meikas, Agne
A1 Krjutškov, Kaarel
A1 Aghajanova, Lusine
A1 Lalitkumar, Parameswaran G
A1 Gemzell-Danielsson, Kristina
A1 Giudice, Linda
A1 Simón, Carlos
A1 Salumets, Andres
AB Previous transcriptome studies of the human endometrium have revealed hundreds of simultaneously up- and down-regulated genes that are involved in endometrial receptivity. However, the overlap between the studies is relatively small, and we are still searching for potential diagnostic biomarkers. Here we perform a meta-analysis of endometrial-receptivity associated genes on 164 endometrial samples (76 from 'pre-receptive' and 88 from mid-secretory, 'receptive' phase endometria) using a robust rank aggregation (RRA) method, followed by enrichment analysis, and regulatory microRNA prediction. We identify a meta-signature of endometrial receptivity involving 57 mRNA genes as putative receptivity markers, where 39 of these we confirm experimentally using RNA-sequencing method in two separate datasets. The meta-signature genes highlight the importance of immune responses, the complement cascade pathway and the involvement of exosomes in mid-secretory endometrial functions. Bioinformatic prediction identifies 348 microRNAs that could regulate 30 endometrial-receptivity associated genes, and we confirm experimentally the decreased expression of 19 microRNAs with 11 corresponding up-regulated meta-signature genes in our validation experiments. The 57 identified meta-signature genes and involved pathways, together with their regulatory microRNAs could serve as promising and sought-after biomarkers of endometrial receptivity, fertility and infertility.
YR 2017
FD 2017-08-30
LK http://hdl.handle.net/10668/11544
UL http://hdl.handle.net/10668/11544
LA en
DS RISalud
RD Apr 6, 2025