RT Journal Article
T1 Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline.
A1 Rybakowska, Paulina
A1 Van Gassen, Sofie
A1 Martorell Marugán, Jordi
A1 Quintelier, Katrien
A1 Saeys, Yvan
A1 Alarcón-Riquelme, Marta E
A1 Marañón, Concepción
K1 Clinical protocol
K1 Flow cytometry/Mass cytometry
K1 Immunology
AB Mass cytometry (MC) is a powerful large-scale immune monitoring technology. To maximize MC data quality, we present a protocol for whole blood analysis together with an R package, Cyto Quality Pipeline (CytoQP), which minimizes the experimental artifacts and batch effects to ensure data reproducibility. We describe the steps to stimulate, fix, and freeze blood samples before acquisition to make them suitable for retrospective studies. We then detail the use of barcoding and reference samples to facilitate multicenter and multi-batch experiments. For complete details on the use and execution of this protocol, please refer to Rybakowska et al. (2021a) and (2021b).
YR 2022
FD 2022-10-31
LK http://hdl.handle.net/10668/22544
UL http://hdl.handle.net/10668/22544
LA en
DS RISalud
RD Apr 12, 2025