RT Journal Article
T1 Ceramide chain length-dependent protein sorting into selective endoplasmic reticulum exit sites
A1 Rodriguez-Gallardo, Sofia
A1 Kurokawa, Kazuo
A1 Sabido-Bozo, Susana
A1 Cortes-Gomez, Alejandro
A1 Ikeda, Atsuko
A1 Zoni, Valeria
A1 Aguilera-Romero, Auxiliadora
A1 Perez-Linero, Ana Maria
A1 Lopez, Sergio
A1 Waga, Miho
A1 Araki, Misako
A1 Nakano, Miyako
A1 Riezman, Howard
A1 Funato, Kouichi
A1 Vanni, Stefano
A1 Nakano, Akihiko
A1 Muñiz, Manuel
K1 Ceramides
K1 Glycosylphosphatidylinositol
K1 Endoplasmic reticulum
K1 Lipids
K1 Membranes
K1 Proteins
K1 Reticulum
K1 Secretory pathway
K1 Ceramidas
K1 Retículo endoplásmico
K1 Lípidos
K1 Proteínas
K1 Reticulum
K1 Vías secretorias
K1 Membranas
K1 Glicosilfosfatidilinositoles
AB Protein sorting in the secretory pathway is crucial to maintain cellular compartmentalization and homeostasis. In addition to coat-mediated sorting, the role of lipids in driving protein sorting during secretory transport is a longstanding fundamental question that still remains unanswered. Here, we conduct 3D simultaneous multicolor high-resolution live imaging to demonstrate in vivo that newly synthesized glycosylphosphatidylinositol-anchored proteins having a very long chain ceramide lipid moiety are clustered and sorted into specialized endoplasmic reticulum exit sites that are distinct from those used by transmembrane proteins. Furthermore, we show that the chain length of ceramide in the endoplasmic reticulum membrane is critical for this sorting selectivity. Our study provides the first direct in vivo evidence for lipid chain length-based protein cargo sorting into selective export sites of the secretory pathway.
PB American Association for the Advancement of Science.
YR 2020
FD 2020-12-11
LK http://hdl.handle.net/10668/3553
UL http://hdl.handle.net/10668/3553
LA en
NO Rodriguez-Gallardo S, Kurokawa K, Sabido-Bozo S, Cortes-Gomez A, Ikeda A, Zoni V, et al. Ceramide chain length-dependent protein sorting into selective endoplasmic reticulum exit sites. Sci Adv. 2020 Dec 11;6(50):eaba8237
DS RISalud
RD Apr 9, 2025