RT Journal Article
T1 Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast.
A1 Jaafar, Mariam
A1 Contreras, Julia
A1 Dominique, Carine
A1 Martín-Villanueva, Sara
A1 Capeyrou, Regine
A1 Vitali, Patrice
A1 Rodriguez-Galan, Olga
A1 Velasco, Carmen
A1 Humbert, Odile
A1 Watkins, Nicholas J
A1 Villalobo, Eduardo
A1 Bohnsack, Katherine E
A1 Bohnsack, Markus T
A1 Henry, Yves
A1 Merhi, Raghida Abou
A1 de-la-Cruz, Jesus
A1 Henras, Anthony K
K1 RNA
K1 RNA folding
K1 Small RNAs
AB Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least understood stages of ribosome biogenesis. It involves the Npa1 complex, a protein module suggested to play a key role in the early structuring of the pre-rRNA. Npa1 displays genetic interactions with the DExD-box protein Dbp7 and interacts physically with the snR190 box C/D snoRNA. We show here that snR190 functions as a snoRNA chaperone, which likely cooperates with the Npa1 complex to initiate compaction of the pre-rRNA in early pre-60S r-particles. We further show that Dbp7 regulates the dynamic base-pairing between snR190 and the pre-rRNA within the earliest pre-60S r-particles, thereby participating in structuring the peptidyl transferase center (PTC) of the large ribosomal subunit.
PB Nature Publishing Group
YR 2021
FD 2021-10-22
LK https://hdl.handle.net/10668/27566
UL https://hdl.handle.net/10668/27566
LA en
NO Jaafar M, Contreras J, Dominique C, Martín-Villanueva S, Capeyrou R, Vitali P, et al. Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast. Nat Commun. 2021 Oct 22;12(1):6153.
DS RISalud
RD Apr 5, 2025