RT Journal Article
T1 L-Arginine metabolism in cardiovascular and renal tissue from hyper- and hypothyroid rats.
A1 Rodriguez-Gomez, Isabel
A1 Moliz, Juan N
A1 Quesada, Andres
A1 Montoro-Molina, Sebastian
A1 Vargas-Tendero, Pablo
A1 Osuna, Antonio
A1 Wangensteen, Rosemary
A1 Vargas, Felix
K1 Hyperthyroidism
K1 aorta
K1 heart kidney
K1 hypothyroidism
K1 l-arginine metabolism
K1 rat
AB This study assessed the effects of thyroid hormones on the enzymes involved in  l-arginine metabolism and the metabolites generated by the different metabolic pathways . Compounds of  l-arginine metabolism were measured in the kidney , heart , aorta , and liver of euthyroid, hyperthyroid , and hypothyroid rats after 6 weeks of treatment . Enzymes studied were NOS isoforms (neuronal [nNOS], inducible [iNOS], and endothelial [eNOS]), arginases I and II, ornithine decarboxylase (ODC), ornithine aminotransferase ( OAT ), and  l-arginine decarboxylase (ADC). Metabolites studied were  l-arginine , l- citrulline , spermidine , spermine , and  l-proline . Kidney heart and aorta levels of eNOS and iNOS were augmented and reduced (P < 0.05, for each tissue and enzyme ) in hyper- and hypothyroid rats , respectively. Arginase I abundance in aorta , heart , and kidney was increased (P < 0.05, for each tissue ) in hyperthyroid rats and was decreased in kidney and aorta of hypothyroid rats (P < 0.05, for each tissue ). Arginase II was augmented in aorta and kidney (P < 0.05, for each tissue ) of hyperthyroid rats and remained unchanged in all organs of hypothyroid rats . The substrate for these enzymes ,  l-arginine , was reduced (P < 0.05, for all tissues ) in hyperthyroid rats . Levels of ODC and spermidine , its product, were increased and decreased (P < 0.05) in hyper- and hypothyroid rats , respectively, in all organs studied. OAT and proline levels were positively modulated by thyroid hormones in liver but not in the other tissues . ADC protein levels were positively modulated by thyroid hormones in all tissues . According to these findings, thyroid hormone treatment positively modulates different  l-arginine metabolic pathways . The changes recorded in the abundance of eNOS, arginases I and II, and ADC protein in renal and cardiovascular tissues may play a role in the hemodynamic and renal manifestations observed in thyroid disorders. Furthermore, the changes in ODC and spermidine might contribute to the changes in cardiac and renal mass observed in thyroid disorders.
PB Frontiers Media SA
YR 2016
FD 2016-03
LK http://hdl.handle.net/10668/9667
UL http://hdl.handle.net/10668/9667
LA en
NO Rodríguez-Gómez I, Moliz JN, Quesada A, Montoro-Molina S, Vargas-Tendero P, Osuna A, et al. L-Arginine metabolism in cardiovascular and renal tissue from hyper- and hypothyroid rats. Exp Biol Med (Maywood). 2016 Mar;241(5):550-6.
DS RISalud
RD Aug 5, 2025