RT Journal Article
T1 Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats.
A1 Alcaide-Hidalgo, Juan María
A1 Romero, Miguel
A1 Duarte, Juan
A1 López-Huertas, Eduardo
K1 ACE
K1 hypertension
K1 peptides
K1 spontaneously hypertensive rats
K1 unfiltered virgin olive oil
AB The low molecular weight peptide composition of virgin olive oil (VOO) is mostly unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract from it. The peptides were separated by size-exclusion using fast protein liquid chromatography, and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences containing between 6 and 9 amino acids and molecular masses ranging 698-1017 Da. Those peptides were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration (IC50)
YR 2020
FD 2020-01-20
LK http://hdl.handle.net/10668/14986
UL http://hdl.handle.net/10668/14986
LA en
DS RISalud
RD May 11, 2025