RT Journal Article
T1 Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs
A1 Casas-Arrojo, Virginia
A1 Arrojo Agudo, Maria de Los Angeles
A1 Cardenas Garcia, Casimiro
A1 Carrillo, Paloma
A1 Perez Manriquez, Claudia
A1 Martinez-Manzanares, Eduardo
A1 Abdala Diaz, Roberto T.
K1 euglena gracilis
K1 polysaccharides
K1 immunomodulatory capacity
K1 antioxidant activity
K1 anticancer capacity and nutraceuticals
K1 heterotrophic cultivation
K1 sulfated polysaccharides
K1 marine polysaccharides
K1 beta-glucan
K1 microalgae
K1 paramylon
K1 growth
K1 beta-1,3-glucan
K1 alpha
K1 algae
AB The present study was carried out to determine the bioactivity of polysaccharides extracted from Euglena gracilis (EgPs). These were characterized by FT-IR and GC-MS. Cytotoxicity analyses (MTT) were performed on healthy human gingival fibroblast cell lines (HGF-1), obtaining an IC50 of 228.66 mu g mL(-1), and cell lines with anticancer activity for colon cancer (HCT-116), breast cancer (MCF-7), human leukemia (U-937, HL-60) and lung cancer (NCI-H460), showing that EgPs have anticancer activity, mainly in HTC-116 cells (IC50 = 26.1 mu g mL(-1)). The immunological assay determined the immunomodulatory capacity of polysaccharides for the production of proinflammatory cytokines IL-6 and TNF-alpha in murine macrophages (RAW 264.7) and TNF-alpha in human monocytes (THP-1). It was observed that the EgPs had a stimulating capacity in the synthesis of these interleukins. The antioxidant capacity of polysaccharides and their biomass were analyzed using the ABTS method (18.30 +/- 0.14% and (5.40 +/- 0.56%, respectively, and the DPPH method for biomass (17.79 +/- 0.57%). We quantitatively profiled HGF-1 proteins by liquid chromatography-tandem mass spectrometry analysis, coupled with 2-plex tandem mass tag labelling, in normal cells. In total, 1346 proteins were identified and quantified with high confidence, of which five were considered to be overexpressed. The data is available through ProteomeXchange, under identifier PXD029076.
PB MDPI
YR 2022
FD 2022-11-02
LK http://hdl.handle.net/10668/21537
UL http://hdl.handle.net/10668/21537
LA en
NO Casas-Arrojo V, Arrojo Agudo MLÁ, Cárdenas García C, Carrillo P, Pérez Manríquez C, Martínez-Manzanares E, et al. Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs. Pharmaceuticals (Basel). 2022 Nov 10;15(11):1379
DS RISalud
RD Apr 7, 2025