TY  - JOUR
AU  - Queipo-Ortuño, Maria Isabel
AU  - Colmenero, Juan de Dios
AU  - Macias, Manuel
AU  - Bravo, Maria Jose
AU  - Morata, Pilar
PY  - 2008
DO  - 10.1128/CVI.00270-07
SN  - 1556-6811
UR  - http://hdl.handle.net/10668/1578
T2  - Clinical and vaccine immunology : CVI
AB  - Real-time PCR is a widely used tool for the diagnosis of many infectious diseases. However, little information exists about the influences of the different factors involved in PCR on the amplification efficiency. The aim of this study was to analyze...
LA  - en
PB  - American Society for Microbiology
KW  - Brucelosis
KW  - ADN bacteriano
KW  - Inmunotransferencia Western
KW  - Electroforesis en gel de poliacrilamida
KW  - Inhibidores enzimáticos
KW  - Inmunoglobulina G
KW  - Reacción en cadena de la polimerasa
KW  - Suero
KW  - Medical Subject Headings::Diseases::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Brucellosis
KW  - Medical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Bacterial
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Electrochemical Techniques::Electrophoresis::Electrophoresis, Polyacrylamide Gel
KW  - Medical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Enzyme Inhibitors
KW  - Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans
KW  - Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin G
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction
KW  - Medical Subject Headings::Anatomy::Hemic and Immune Systems::Blood::Serum
KW  - Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay::Immunoblotting::Blotting, Western
TI  - Preparation of bacterial DNA template by boiling and effect of immunoglobulin G as an inhibitor in real-time PCR for serum samples from patients with brucellosis.
TY  - research article
ER  -