RT Journal Article
T1 Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota.
A1 Garzarella, Emanuele Ugo
A1 Navajas-Porras, Beatriz
A1 Perez-Burillo, Sergio
A1 Ullah, Hammad
A1 Esposito, Cristina
A1 Santarcangelo, Cristina
A1 Hinojosa-Nogueira, Daniel
A1 Pastoriza, Silvia
A1 Zaccaria, Vincenzo
A1 Xiao, Jianbo
A1 Rufian-Henares, Jose Angel
A1 Daglia, Maria
K1 Antioxidant capacity
K1 Gut microbiota
K1 In vitro digestion
K1 In vitro fermentation
K1 Multi dynamic extraction
K1 Poplar-type propolis
K1 Short-chain fatty acids
AB A large body of evidence suggests that propolis exerts antioxidant, anti-inflammatory, and antimicrobial activities, mostly ascribed to its polyphenol content. Growing evidence suggests that propolis could modulate gut microbiota exerting a positive impact on several pathological conditions. The aim of this study was to determine the in vitro impact of a poplar-type propolis extract with a standardized polyphenol content, on the composition and functionality of gut microbiota obtained from fecal material of five different donors (healthy adults, and healthy, obese, celiac, and food allergic children). The standardized polyphenol mixture was submitted to a simulated in vitro digestion-fermentation process, designed to mimic natural digestion in the human oral, gastric, and intestinal chambers. The antioxidant profile of propolis before and after the digestion-fermentation process was determined. 16 S rRNA amplicon next-generation sequencing (NGS) was used to test the effects on the gut microbiota of propolis extract. The profile of the short-chain fatty acids (SCFA) produced by the microbiota was also investigated through a chromatographic method coupled with UV detection. In vitro digestion and fermentation induced a decrease in the antioxidant profile of propolis (i.e., decrease of total polyphenol content, antiradical and reducing activities). Propolis fermentation exhibited a modulatory effect on gut microbiota composition and functionality of healthy and diseased subjects increasing the concentration of SCFA. Overall, these data suggest that propolis might contribute to gut health and could be a candidate for further studies in view of its use as a prebiotic ingredient.
PB Elsevier Masson
YR 2022
FD 2022-02-23
LK http://hdl.handle.net/10668/22041
UL http://hdl.handle.net/10668/22041
LA en
NO Garzarella EU, Navajas-Porras B, Pérez-Burillo S, Ullah H, Esposito C, Santarcangelo C,  et al.  Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota. Biomed Pharmacother. 2022 Apr;148:112759.
DS RISalud
RD Sep 26, 2025