%0 Journal Article
%A Sanbonmatsu-Gámez, Sara
%A Pérez-Ruiz, Mercedes
%A Collao, Ximena
%A Sánchez-Seco, María Paz
%A Morillas-Márquez, Francisco
%A Rosa-Fraile, Manuel de la
%A Navarro-Marí, José María
%A Tenorio, Antonio
%T Toscana virus in Spain
%D 2005
%@ 1080-6040
%U http://hdl.handle.net/10668/608
%X Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.
%K Animales
%K Anticuerpos Antivirales
%K Femenino
%K Humanos
%K Inmunoglobulina G
%K Insectos Vectores
%K Masculino
%K Phlebotomus
%K Fiebre por Flebótomos
%K Filogenia
%K Prevalencia
%K Virus de Nápoles de la Fiebre de la Mosca de los Arenales
%K Análisis de Secuencia de ADN
%K España
%K Proteínas Virales
%~