%0 Journal Article
%A Puig, Noemi
%A Paiva, Bruno
%A Lasa, Marta
%A Burgos, Leire
%A Perez, Jose J
%A Merino, Juana
%A Moreno, Cristina
%A Vidriales, Maria-Belen
%A Toboso, Dolores Gómez
%A Cedena, Maria-Teresa
%A Ocio, Enrique M
%A Lecumberri, Ramon
%A García de Coca, Alfonso
%A Labrador, Jorge
%A Gonzalez, Maria-Esther
%A Palomera, Luis
%A Gironella, Mercedes
%A Cabañas, Valentin
%A Casanova, Maria
%A Oriol, Albert
%A Krsnik, Isabel
%A Pérez-Montaña, Albert
%A de la Rubia, Javier
%A de la Puerta, Jose-Enrique
%A de Arriba, Felipe
%A Prosper, Felipe
%A Martinez-Lopez, Joaquin
%A Lecrevisse, Quentin
%A Verde, Javier
%A Mateos, Maria-Victoria
%A Lahuerta, Juan-Jose
%A Orfao, Alberto
%A San Miguel, Jesús F
%T Flow cytometry for fast screening and automated risk assessment in systemic light-chain amyloidosis.
%D 2018
%U https://hdl.handle.net/10668/26671
%X Early diagnosis and risk stratification are key to improve outcomes in light-chain (AL) amyloidosis. Here we used multidimensional-flow-cytometry (MFC) to characterize bone marrow (BM) plasma cells (PCs) from a series of 166 patients including newly-diagnosed AL amyloidosis (N = 94), MGUS (N = 20) and multiple myeloma (MM, N = 52) vs. healthy adults (N = 30). MFC detected clonality in virtually all AL amyloidosis (99%) patients. Furthermore, we developed an automated risk-stratification system based on BMPCs features, with independent prognostic impact on progression-free and overall survival of AL amyloidosis patients (hazard ratio: ≥ 2.9;P ≤ .03). Simultaneous assessment of the clonal PCs immunophenotypic protein expression profile and the BM cellular composition, mapped AL amyloidosis in the crossroad between MGUS and MM; however, lack of homogenously-positive CD56 expression, reduction of B-cell precursors and a predominantly-clonal PC compartment in the absence of an MM-like tumor PC expansion, emerged as hallmarks of AL amyloidosis (ROC-AUC = 0.74;P 
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