RT Journal Article
T1 A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues.
A1 Ribeiro, Marcelo C
A1 Rivera-Arbelaez, Jose M
A1 Cofiño-Fabres, Carla
A1 Schwach, Verena
A1 Slaats, Rolf H
A1 Ten Den, Simone A
A1 Vermeul, Kim
A1 van den Berg, Albert
A1 Perez-Pomares, Jose M
A1 Segerink, Loes I
A1 Guadix, Juan A
A1 Passier, Robert
K1 cardiac performance
K1 contractile force
K1 engineered heart tissues
K1 hPSC-CMs
K1 serum-free
K1 versatile platform
AB Cardiomyocytes derived from human pluripotent stem cells (hPSC-CMs) hold a great potential as human in vitro models for studying heart disease and for drug safety screening. Nevertheless, their associated immaturity relative to the adult myocardium limits their utility in cardiac research. In this study, we describe the development of a platform for generating three-dimensional engineered heart tissues (EHTs) from hPSC-CMs for the measurement of force while under mechanical and electrical stimulation. The modular and versatile EHT platform presented here allows for the formation of three tissues per well in a 12-well plate format, resulting in 36 tissues per plate. We compared the functional performance of EHTs and their histology in three different media and demonstrated that tissues cultured and maintained in maturation medium, containing triiodothyronine (T3), dexamethasone, and insulin-like growth factor-1 (TDI), resulted in a higher force of contraction, sarcomeric organization and alignment, and a higher and lower inotropic response to isoproterenol and nifedipine, respectively. Moreover, in this study, we highlight the importance of integrating a serum-free maturation medium in the EHT platform, making it a suitable tool for cardiovascular research, disease modeling, and preclinical drug testing.
SN 2075-4426
YR 2022
FD 2022-02-01
LK http://hdl.handle.net/10668/21372
UL http://hdl.handle.net/10668/21372
LA en
DS RISalud
RD Apr 18, 2025