RT Journal Article
T1 Pro-Inflammatory Cytokines at Ultra-Low Dose Exert Anti-Inflammatory Effect In Vitro: A Possible Mode of Action Involving Sub-Micron Particles?
A1 Floris, Ilaria
A1 Rose, Thorsten
A1 Collado Rojas, Juan Antonio
A1 Appel, Kurt
A1 Roesch, Camille
A1 Lejeune, Beatrice
K1 Micro-immunotherapy
K1 Ultra-low doses
K1 Anti-inflammatory unitary medicines
K1 Sub-micron particles
K1 Inmunoterapia
K1 Antiinflamatorios
K1 Hormesis
K1 Citocinas
AB Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) are pro-inflammatory cytokines involved in acute and chronic inflammatory diseases. Indeed, immunotherapy blocking these 2 cytokines has been developed. Micro-immunotherapy (MI) also uses ultra-low doses (ULD) of pro-inflammatory cytokines, impregnated on lactose-sucrose pillules, to counteract their overexpression. The study has been conducted with 2 objectives: examine the anti-inflammatory effect in vitro and the capacity of 2 unitary medicines, TNF-α (27 CH) and IL-1β (27 CH), to reduce the secretion of TNF-α in human primary monocytes and THP-1 cells differentiated with phorbol-12-myristate-13-acetate, after lipopolysaccharide (LPS) exposure; then, investigate the presence of particles possibly containing starting materials using tunable resistive pulse sensing technique. The results show that the unitary medicines, tested at 3 pillules concentrations (5.5, 11 and 22 mM), have reduced the secretion of TNF-α in both models by about 10-20% vs. vehicle control, depending on concentration. In this exploratory study, particles (150-1000 nm) have been detected in MI ULD-impregnated pillules and a hypothesis for MI medicines mode of action has been proposed. Conscious that more evaluations are necessary, authors are cautious in the conclusions because the findings described in the study are still limited, and future investigations may lead to different hypothesis.
PB SAGE Publishing
YR 2020
FD 2020-10-21
LK http://hdl.handle.net/10668/3599
UL http://hdl.handle.net/10668/3599
LA en
NO Floris I, Rose T, Collado Rojas JA, Appel K, Roesch C, Lejeune B. Pro-Inflammatory Cytokines at Ultra-Low Dose Exert Anti-Inflammatory Effect In Vitro: A Possible Mode of Action Involving Sub-Micron Particles? Dose Response. 2020 Oct 21;18(4):1559325820961723
DS RISalud
RD Apr 20, 2025