RT Journal Article
T1 A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome.
A1 Chamoun, Betty
A1 Caraben, Anna
A1 Torres, Irina B
A1 Sellares, Joana
A1 Jimenez, Raquel
A1 Toapanta, Nestor
A1 Cidraque, Ignacio
A1 Gabaldon, Alejandra
A1 Perello, Manel
A1 Gonzalo, Ricardo
A1 O'Valle, Francisco
A1 Moreso, Francesc
A1 Seron, Daniel
K1 biopsies
K1 borderline changes
K1 interstitial fibrosis and tubular atrophy
K1 microarrays
K1 rejection
K1 renal transplantation
K1 transcriptomics
AB Rejection-associated gene expression has been characterized in renal allograft biopsies for cause. The aim is to evaluate rejection gene expression in subclinical rejection and in biopsies with borderline changes or interstitial fibrosis and tubular atrophy (IFTA). We included 96 biopsies. Most differentially expressed genes between normal surveillance biopsies (n = 17) and clinical rejection (n = 12) were obtained. A rejection-associated gene (RAG) score was defined as its geometric mean. The following groups were considered: (a) subclinical rejection (REJ-S, n = 6); (b) borderline changes in biopsies for cause (BL-C, n = 13); (c) borderline changes in surveillance biopsies (BL-S, n = 12); (d) IFTA in biopsies for cause (IFTA-C, n = 20); and (e) IFTA in surveillance biopsies (IFTA-S, n = 16). The outcome variable was death-censored graft loss or glomerular filtration rate decline ≥ 30 % at 2 years. A RAG score containing 109 genes derived from normal and clinical rejection (area under the curve, AUC = 1) was employed to classify the study groups. A positive RAG score was observed in 83% REJ-S, 38% BL-C, 17% BL-S, 25% IFTA-C, and 5% IFTA-S. A positive RAG score was an independent predictor of graft outcome from histological diagnosis (hazard ratio: 3.5 and 95% confidence interval: 1.1-10.9; p = 0.031). A positive RAG score predicts graft outcome in surveillance and for cause biopsies with a less severe phenotype than clinical rejection.
PB MDPI AG
YR 2020
FD 2020-11-03
LK http://hdl.handle.net/10668/16557
UL http://hdl.handle.net/10668/16557
LA en
NO Chamoun B, Caraben A, Torres IB, Sellares J, Jiménez R, Toapanta N, et al. A Rejection Gene Expression Score in Indication and Surveillance Biopsies Is Associated with Graft Outcome. Int J Mol Sci. 2020 Nov 3;21(21):8237.
DS RISalud
RD May 9, 2025