RT Conference Proceedings
T1 Identification and Isolation of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients through the Identification of T-Cells Capable to Establish Stable Interactions with Leukemic Cells
A1 Garcia-Guerrero, Estefania
A1 Sanchez-Abarca, Luis I.
A1 Domingo, Esther
A1 Ramos, Teresa
A1 Antonio Bejarano-Garcia, Jose
A1 Gonzalez-Campos, Jose A.
A1 Caballero-Velazquez, Teresa
A1 Perez-Simon, Jose A.
K1 Humans
K1 T-Lymphocytes, Cytotoxic
K1 Lymphocytes, Tumor-Infiltrating
K1 Melanoma
K1 Bone Marrow
K1 Feasibility Studies
AB Autologous adoptive T cell therapies, based on the use of tumor infiltrating lymphocytes (TILs), have made great progress in recent years for the treatment of solid tumors, especially melanoma. However, further work is needed to isolate tumor-reactive T cells among patients diagnosed with hematologic malignancies. The dynamics of the interaction between T cells and antigen presenting cells (APC) dictate the quality of the immune responses. While stable joints between target cells and T lymphocytes lead to the induction of T cell activation and immune response, brief contacts contribute to the induction of immune-tolerance. Taking advantage of the strong interaction between target cell and activated T-cells, we show the feasibility to identify and isolate tumor-specific cytotoxic T lymphocytes (CTLs) from acute myeloid leukemia (AML) patients. Using this approach, CTLs stably bound through T cell receptor to tumor cells (doublet forming T-cells) can be identified in peripheral blood and bone marrow and subsequently selected and isolated by FACS-based cell sorting.
PB Amer soc hematology
SN 0006-4971
YR 2021
FD 2021-06-01
LK https://hdl.handle.net/10668/27810
UL https://hdl.handle.net/10668/27810
LA en
NO Estefania Garcia-Guerrero, Luis I. Sanchez-Abarca, Esther Domingo, Teresa Ramos, Jose Antonio Bejarano-García, Jose A Gonzalez-Campos, et al. Identification and Isolation of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients through the Identification of T-Cells Capable to Establish Stable Interactions with Leukemic Cells, Blood, 2018, (132), Supplement 1, 3336.
DS RISalud
RD Apr 12, 2025