RT Journal Article
T1 Effects of Lifestyle Intervention in Tissue-Specific Lipidomic Profile of Formerly Obese Mice.
A1 Dahdah, Norma
A1 Gonzalez-Franquesa, Alba
A1 Samino, Sara
A1 Gama-Perez, Pau
A1 Herrero, Laura
A1 Perales, Jose Carlos
A1 Yanes, Oscar
A1 Malagon, Maria Del Mar
A1 Garcia-Roves, Pablo Miguel
K1 Diet composition
K1 Energy intake
K1 Exercise
K1 Gastrocnemius
K1 Hypothalamus
K1 Lipidomics
K1 Liver
K1 Plasticity
K1 Tissue-specific
K1 White adipose tissue
AB Lipids are highly diverse in their composition, properties and distribution in different biological entities. We aim to establish the lipidomes of several insulin-sensitive tissues and to test their plasticity when divergent feeding regimens and lifestyles are imposed. Here, we report a proton nuclear magnetic resonance (1H-NMR) study of lipid abundance across 4 tissues of C57Bl6J male mice that includes the changes in the lipid profile after every lifestyle intervention. Every tissue analysed presented a specific lipid profile irrespective of interventions. Glycerolipids and fatty acids were most abundant in epididymal white adipose tissue (eWAT) followed by liver, whereas sterol lipids and phosphoglycerolipids were highly enriched in hypothalamus, and gastrocnemius had the lowest content in all lipid species compared to the other tissues. Both when subjected to a high-fat diet (HFD) and after a subsequent lifestyle intervention (INT), the lipidome of hypothalamus showed no changes. Gastrocnemius and liver revealed a pattern of increase in content in many lipid species after HFD followed by a regression to basal levels after INT, while eWAT lipidome was affected mainly by the fat composition of the administered diets and not their caloric density. Thus, the present study demonstrates a unique lipidome for each tissue modulated by caloric intake and dietary composition.
PB MDPI
YR 2021
FD 2021-03-28
LK http://hdl.handle.net/10668/17657
UL http://hdl.handle.net/10668/17657
LA en
NO Dahdah N, Gonzalez-Franquesa A, Samino S, Gama-Perez P, Herrero L, Perales JC, et al. Effects of Lifestyle Intervention in Tissue-Specific Lipidomic Profile of Formerly Obese Mice. Int J Mol Sci. 2021 Apr 1;22(7):3694
DS RISalud
RD Apr 6, 2025