RT Journal Article
T1 Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts
A1 Toledano-Osorio, Manuel
A1 Manzano-Moreno, Francisco J.
A1 Toledano, Manuel
A1 Medina-Castillo, Antonio L.
A1 Costela-Ruiz, Victor J.
A1 Ruiz, Concepción
A1 Osorio, Raquel
K1 CD markers
K1 Doxycycline
K1 Antigenic phenotype
K1 Osteoblasts
K1 Membrane
K1 Alkaline phosphatase
K1 Flow cytometry
K1 Bone regeneration
K1 Cell proliferation
K1 Periodontal disease
K1 Immunomodulation
K1 Antígenos CD
K1 Doxiciclina
K1 Osteoblastos
K1 Fosfatasa alcalina
K1 Citometría de flujo
K1 Regeneración ósea
K1 Proliferación celular
K1 Enfermedades periodontales
K1 Inmunomodulación
K1 Membranas
AB Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts' proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (p < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease.
PB MDPI
YR 2021
FD 2021-03-28
LK http://hdl.handle.net/10668/4443
UL http://hdl.handle.net/10668/4443
LA en
NO Toledano-Osorio M, Manzano-Moreno FJ, Toledano M, Medina-Castillo AL, Costela-Ruiz VJ, Ruiz C, et al. Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts. Polymers. 2021 Mar 28;13(7):1063
DS RISalud
RD Apr 19, 2025