RT Journal Article
T1 Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases.
A1 Alonso-Rodríguez, Noelia
A1 Martínez-Lirola, Miguel
A1 Sánchez, M Luisa
A1 Herranz, Marta
A1 Peñafiel, Teresa
A1 Bonillo, Magdalena del Carmen
A1 Gonzalez-Rivera, Milagros
A1 Martínez, Juan
A1 Cabezas, Teresa
A1 Diez-García, Luis Felipe
A1 Bouza, Emilio
A1 García de Viedma, Darío
K1 Técnicas de Tipificación Bacteriana
K1 Análisis por Conglomerados
K1 Dermatoglifos de ADN
K1 ADN bacteriano
K1 Genotipo
K1 Secuencias Repetitivas Esparcidas
K1 Repeticiones de Minisatélite
K1 epidemiología molecular
K1 Mycobacterium tuberculosis
K1 Reacción en Cadena de la Polimerasa
K1 Polimorfismo de Longitud del Fragmento de Restricción
K1 Tuberculosis
AB The use of molecular tools for genotyping Mycobacterium tuberculosis isolates in epidemiological surveys in order to identify clustered and orphan strains requires faster response times than those offered by the reference method, IS6110 restriction fragment length polymorphism (RFLP) genotyping. A method based on PCR, the mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping technique, is an option for fast fingerprinting of M. tuberculosis, although precise evaluations of correlation between MIRU-VNTR and RFLP findings in population-based studies in different contexts are required before the methods are switched. In this study, we evaluated MIRU-VNTR genotyping (with a set of 15 loci [MIRU-15]) in parallel to RFLP genotyping in a 39-month universal population-based study in a challenging setting with a high proportion of immigrants. For 81.9% (281/343) of the M. tuberculosis isolates, both RFLP and MIRU-VNTR types were obtained. The percentages of clustered cases were 39.9% (112/281) and 43.1% (121/281) for RFLP and MIRU-15 analyses, and the numbers of clusters identified were 42 and 45, respectively. For 85.4% of the cases, the RFLP and MIRU-15 results were concordant, identifying the same cases as clustered and orphan (kappa, 0.7). However, for the remaining 14.6% of the cases, discrepancies were observed: 16 of the cases clustered by RFLP analysis were identified as orphan by MIRU-15 analysis, and 25 cases identified as orphan by RFLP analysis were clustered by MIRU-15 analysis. When discrepant cases showing subtle genotypic differences were tolerated, the discrepancies fell from 14.6% to 8.6%. Epidemiological links were found for 83.8% of the cases clustered by both RFLP and MIRU-15 analyses, whereas for the cases clustered by RFLP or MIRU-VNTR analysis alone, links were identified for only 30.8% or 38.9% of the cases, respectively. The latter group of cases mainly comprised isolates that could also have been clustered, if subtle genotypic differences had been tolerated. MIRU-15 genotyping seems to be a good alternative to RFLP genotyping for real-time interventional schemes. The correlation between MIRU-15 and IS6110 RFLP findings was reasonable, although some uncertainties as to the assignation of clusters by MIRU-15 analysis were identified.
PB American Society for Microbiology
SN 0095-1137
YR 2009
FD 2009-05-20
LK http://hdl.handle.net/10668/631
UL http://hdl.handle.net/10668/631
LA en
NO Alonso-Rodriguez N, Martínez-Lirola M, Sánchez ML, Herranz M, Peñafiel T, Bonillo M del C, et al. Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases. J. Clin. Microbiol. 2009 Jul; 47(7):2026-32
DS RISalud
RD Apr 4, 2025