RT Journal Article
T1 Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus
A1 Caro-Astorga, Joaquín
A1 Frenzel, Elrike
A1 Perkins, James R.
A1 Álvarez-Mena, Ana
A1 de Vicente, Antonio
A1 Ranea, Juan A. G.
A1 Kuipers, Oscar P.
A1 Romero, Diego
K1 Biofilms
K1 Next-generation sequencing
K1 Bacillus cereus
K1 Biopelículas
K1 Secuenciación de nucleótidos de alto rendimiento
AB Biofilm formation is a strategy of many bacterial species to adapt to a variety of stresses and has become a part of infections, contaminations, or beneficial interactions. In this study, we demonstrate that profound physiological changes permit Bacillus cereus to switch from a floating to a sessile lifestyle, to undergo further maturation of the biofilm and to differentiate into the offensive or defensive features. We report that floating and biofilm cells are populations that differentiate metabolically, with members of each subpopulation developing different branches of certain metabolic pathways. Secondly, biofilm populations rearrange nucleotides, sugars, amino acids, and energy metabolism. Thirdly, this metabolic rearrangement coexists with: the synthesis of the extracellular matrix, sporulation, reinforcement of the cell wall, activation of the ROS detoxification machinery and production of secondary metabolites. This strategy contributes to defend biofilm cells from competitors. However, floating cells maintain a fermentative metabolic status that ensures a higher aggressiveness against hosts, evidenced by the production of toxins. The maintenance of the two distinct subpopulations is an effective strategy to face different environmental conditions found in the life styles of B. cereus.
PB Springer Nature
YR 2020
FD 2020-01-15
LK http://hdl.handle.net/10668/4026
UL http://hdl.handle.net/10668/4026
LA en
NO Caro-Astorga J, Frenzel E, Perkins JR, Álvarez-Mena A, de Vicente A, Ranea JAG, et al. Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus. NPJ Biofilms Microbiomes. 2020 Jan 15;6:3
DS RISalud
RD Apr 6, 2025