RT Journal Article
T1 Characterization of Adult Stem/Progenitor Cell Populations From Bone Marrow in a Three-Dimensional Collagen Gel Culture System.
A1 Claros, Silvia
A1 Rodríguez-Losada, Noela
A1 Cruz, Encarnación
A1 Guerado, Enrique
A1 Becerra, José
A1 Andrades, José A
K1 Desmineralización Ósea Patológica
K1 Molécula de Adhesión Celular del Leucocito Activado
K1 Integrina alfa1
K1 Integrina alfa2
K1 Antígenos CD29
K1 Colágeno Tipo I
K1 Antígenos CD34
AB Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells which are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and non-adherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4 and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent, hematopoietic, mesenchymal and endothelial specific markers in vitro and can undergo osteogenic differentiation in vivo.
PB Cognizant Communication Corporation
SN 0963-6897
YR 2012
FD 2012-04-02
LK http://hdl.handle.net/10668/735
UL http://hdl.handle.net/10668/735
LA es
NO Claros, Silvia; Rodríguez-Losada, Noela; Cruz, Encarnación; Guerado, Enrique; Becerra, José; Andrades, José A. Characterization of adult stem/progenitor cell populations from bone marrow in a three-dimensional collagen gel culture system. Cell Transplantation. 2012;21(9):2021–2032
NO JOURNAL ARTICLE;
DS RISalud
RD Apr 6, 2025